Abstract

The long-term goal of this research program is to obtain fundamental new information on the mechanism of steroid hormone action in the mammalian uterus. The previous results from this laboratory have established the utility of the decidualized hamster uterus for experimental studies of receptor regulation and specific protein synthesis in different uterine cell types, i.e., the decidual cell and uterine smooth muscle cell. The current application proposes to continue this research program for a five-year period with the following specific aims: 1. to determine the relative importance of estrogen (E)+ progesterone (P) in the regulation of steroid receptors in the deciduoma and myometrium. What is the critical E/P ratio for determining up and down regulation of estrogen and progesterone receptors in different uterine cell types? 2. to identify cell-specific and hormone-specific proteins in decidual cells. 3. to develop specific antibodies to decidual marker proteins. 4. to study the specific effects of steroid hormones (E and P), cyclic nucleotides (cAMP, cGMP) and prostaglandins on steroid receptors and macromolecular synthesis in decidual cells. These studies will provide information relevant to the treatment of hormone-dependent tumors and the development of new contraceptive techniques.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD018712-04
Application #
3315851
Study Section
Biochemical Endocrinology Study Section (BCE)
Project Start
1983-09-01
Project End
1989-11-30
Budget Start
1986-12-01
Budget End
1987-11-30
Support Year
4
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
Texas Tech University
Department
Type
Schools of Medicine
DUNS #
609980727
City
Lubbock
State
TX
Country
United States
Zip Code
79430

  Publications

Selcer, K W; Lin, G X; Beale, E G et al. (1991) Serum corticosteroid-binding globulin (CBG) and hepatic CBG mRNA relationships during hamster pregnancy: contribution of decidualization. Biol Reprod 44:185-90
Lin, G X; Selcer, K W; Beale, E G et al. (1990) Characterization of the corticosteroid-binding globulin messenger ribonucleic acid response in the pregnant hamster. Endocrinology 127:1934-40
Selcer, K W; Leavitt, W W (1989) Characterization of the corticosteroid-binding globulin response to decidualization in the hamster. Endocrinology 125:976-83
Selcer, K W; Leavitt, W W (1988) Hamster uterine tissues accumulate corticosteroid-binding globulin during decidualization. Biol Reprod 39:592-602
Okulicz, W C; Leavitt, W W (1988) Binding and biologic activity of diethylstilbestrol in the hamster: influence of a serum component on estrogen receptor binding and estrogenic activity. J Steroid Biochem 31:371-5
Chilton, B S; Williams, N D; Cobb, A D et al. (1987) Ligand-receptor dissociation: a potential mechanism for the attenuation of estrogen action in the juvenile rabbit uterus. Endocrinology 120:750-7
Cobb, A D; Leavitt, W W (1987) Characterization of nuclear acceptor sites for mammalian progesterone receptor: comparison with the chick oviduct system. Gen Comp Endocrinol 67:214-20
Renegar, R H; Cobb, A D; Leavitt, W W (1987) Immunocytochemical localization of relaxin in the golden hamster (Mesocricetus auratus) during the last half of gestation. Biol Reprod 37:925-34
Gray, G O; Leavitt, W W (1987) RU486 is not an antiprogestin in the hamster. J Steroid Biochem 28:493-7
Leavitt, W W; Rundle, S; Thompson, K et al. (1987) Decidual cell function: evidence for a role in the regulation of serum CBG and a 60 kDa protein during early pregnancy in the hamster. Adv Exp Med Biol 230:187-205

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