Problems of both population control, and diagnosis and treatment of infertility, are of major importance to today's society. Integral to caring for such matters are assay systems for pituitary and gonadal hormones that control reproductive functions. Currently, such assays usually require 2 to 4 days to perform. Recently, by optimizing conventional competitive binding immunoassay techniques for human luteinizing hormone (hLH), we have developed an assay that requires only 2 hours to perform. This assay is currently in routine use in our hospital. We now propose to develop so-called """"""""two-site"""""""" noncompetitive assays for human LH, follicle stimulating hormone, and chorionic gonadotropins that can be performed in 15 minutes. Such assays would utilize highly selected monoclonal antibodies labeled with 125I (or later enzyme labeled) as a first reagent. This reagent is used in """"""""great excess,"""""""" permitting rapid reaction. A second polyclonal antibody directed against multiple other determinants on the hormone is used to separate labeled """"""""bound"""""""" monoclonal antibody from """"""""free."""""""" Such """"""""short turn-around"""""""" assays will permit evaluation of a patient during the initial clinic visit, as well as determination of the ovulatory surge during its occurrence. Such methods would be widely applicable and usable in Obstetrical/Gynecological centers and Endocrine centers treating infertility, and for the control of fertility.

Project Start
1984-07-01
Project End
1987-06-30
Budget Start
1986-07-01
Budget End
1987-06-30
Support Year
3
Fiscal Year
1986
Total Cost
Indirect Cost
Name
University of Utah
Department
Type
Schools of Medicine
DUNS #
City
Salt Lake City
State
UT
Country
United States
Zip Code
84112
Grover, S; Odell, W D (1993) Characterization of the 48.5 kDa chorionic gonadotropin-like protein from Xanthomonas maltophilia. Endocr Res 19:147-62
Grover, S; Odell, W D (1992) Partial characterization of the 30 kD Ig-binding protein from Pseudomonas maltophilia. Biochem Biophys Res Commun 182:1075-81
Grover, S; Griffin, J; Odell, W D (1991) Ultrasensitive, specific, two-antibody immunoradiometric assay that detects free alpha subunits of glycoprotein hormones in blood of nonpregnant humans. Clin Chem 37:2069-75
Grover, S; McGee, Z A; Odell, W D (1991) Isolation of a 30 kDa immunoglobulin binding protein from Pseudomonas maltophilia. J Immunol Methods 141:187-97
Grover, S; McGee, Z A; Odell, W D (1991) Isolation of a 48.5-kDa membrane protein from Pseudomonas maltophilia which exhibits immunologic cross-reaction to the beta-subunit of human chorionic gonadotropin. Endocrinology 128:3096-104
Odell, W D; Griffin, J; Bashey, H M et al. (1990) Secretion of chorionic gonadotropin by cultured human pituitary cells. J Clin Endocrinol Metab 71:1318-21
Odell, W D; Griffin, J (1989) Pulsatile secretion of chorionic gonadotropin during the normal menstrual cycle. J Clin Endocrinol Metab 69:528-32
Odell, W D; Griffin, J (1987) Two-monoclonal-antibody ""sandwich""-type assay of human lutropin, with no cross reaction with choriogonadotropin. Clin Chem 33:1603-7
Odell, W D; Griffin, J (1987) Pulsatile secretion of human chorionic gonadotropin in normal adults. N Engl J Med 317:1688-91
Griffin, J; Odell, W D (1987) Ultrasensitive immunoradiometric assay for chorionic gonadotropin which does not cross-react with luteinizing hormone nor free beta chain of hCG and which detects hCG in blood of non-pregnant humans. J Immunol Methods 103:275-83

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