Plasma membrane glycoproteins and the fine-structure of uterine epithelial cell surface are two of the most likely sites of ultimate control of embryo implantation in mammals. Yet very little is known about the cell biology of the uterine epithelium, owing to the lack of an adequate in vitro system. To this end, changes will be investigated in a major class of highly negatively charged glycoproteins, the proteoglycans, and in the surface ultrastructure of mouse uterine epithelium in response to progesterone and estradiol given in vivo. This information will then be used to evaluate the response of isolated uterine epithelium to steroids in vitro, using a culture method in which isolated sheets of epithelium are maintained as sealed vesicles. The cultures will be compared in the presence and absence of endometrial stromal cells, which appear to be required for steroid action on implantation in vivo.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
3R01HD019530-03S1
Application #
3316838
Study Section
Reproductive Biology Study Section (REB)
Project Start
1985-07-01
Project End
1988-09-30
Budget Start
1988-04-01
Budget End
1988-09-30
Support Year
3
Fiscal Year
1988
Total Cost
Indirect Cost
Name
Oregon State University
Department
Type
Schools of Arts and Sciences
DUNS #
053599908
City
Corvallis
State
OR
Country
United States
Zip Code
97339
Chai, L; Morris, J E (1999) Heparan sulfate in the inner limiting membrane of embryonic chicken retina binds basic fibroblast growth factor to promote axonal outgrowth. Exp Neurol 160:175-85
Potter, S W; Gaza, G; Morris, J E (1996) Estradiol induces E-cadherin degradation in mouse uterine epithelium during the estrous cycle and early pregnancy. J Cell Physiol 169:1-14
Doss, R P; Potter, S W; Soeldner, A H et al. (1995) Adhesion of germlings of Botrytis cinerea. Appl Environ Microbiol 61:260-5
Chai, L; Morris, J E (1994) Distribution of heparan sulfate proteoglycans in embryonic chicken neural retina and isolated inner limiting membrane. Curr Eye Res 13:669-77
Morris, J E; Gaza, G; Potter, S W (1994) Specific stimulation of basal lamina heparan sulfate proteoglycan in mouse uterine epithelium by Matrigel and by transforming growth factor-beta 1. In Vitro Cell Dev Biol Anim 30A:120-8
Astrahantseff, K N; Morris, J E (1994) Estradiol-17 beta stimulates proliferation of uterine epithelial cells cultured with stromal cells but not cultured separately. In Vitro Cell Dev Biol Anim 30A:769-76
Morris, J E (1993) Proteoglycans and the modulation of cell adhesion by steric exclusion. Dev Dyn 196:246-51
Potter, S W; Morris, J E (1992) Changes in histochemical distribution of cell surface heparan sulfate proteoglycan in mouse uterus during the estrous cycle and early pregnancy. Anat Rec 234:383-90
Plytycz, B; Potter, S W; Cohen, N et al. (1988) In vitro fusion of newt macrophages. J Exp Zool 246:319-23
Morris, J E; Potter, S W; Gaza-Bulseco, G (1988) Estradiol induces an accumulation of free heparan sulfate glycosaminoglycan chains in uterine epithelium. Endocrinology 122:242-53

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