The proposed studies are designed to examine the long term effects of otitis media with effusion acquired during infancy and early childhood. Thses studies will be carried out longitudinally in cohorts of normal neonates and children and will involve careful clinical evaluation, followed by tympanocentesis in appropriate subjects. Sequentially collected samples of serum, heparinized blood, and middle ear aspirates will be examined for the presence of selected bacteria and viruses and for microbial specific antigens. Specific studies will be undertaken to determine the characteristics and functional levels of specific immunologic reactivity in the middle ear. The release of pharmacologic mediators by polymorphonuclear leukocytes and other inflammatory cells observed in the middle ear excudates will also be studied. The laboratory procedures will include routine and some sophisticated microbiologic and immunologic tests. All are currently in use in our laboratories. It is anticipated that the results emanating from these investigations will define the natural history of otitis media in infancy relative to the nature of innate or infection-induced immunologic reactivity, the characteristics of bacterial-viral interation, or the activation of other non-immunologic mechanisms (such as the oxidative metabolites of polymorphonuclear leukocytes) in the middle ear cavity. The studies should determine the factors which predispose to the development of recurrent otitis media or different forms of chronic or persistent otitis media with serous, mucoid or purulent effusions in infancy and childhood. These studies would be essential to the long range goals of management of childhood otitis media namely; the appropriate antimicrobial therapy and eventual development of effective measures of prevention.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
1R01HD019679-01A1
Application #
3317113
Study Section
Hearing Research Study Section (HAR)
Project Start
1986-01-01
Project End
1989-12-31
Budget Start
1986-01-01
Budget End
1986-12-31
Support Year
1
Fiscal Year
1986
Total Cost
Indirect Cost
Name
Children's Hospital (Buffalo)
Department
Type
DUNS #
City
Buffalo
State
NY
Country
United States
Zip Code
14222
Faden, H; Duffy, L; Foels, T et al. (1996) Adherence of nontypeable Haemophilus influenzae to respiratory epithelium of otitis-prone and normal children. Ann Otol Rhinol Laryngol 105:367-70
Kodama, H; Faden, H; Harabuchi, Y et al. (1996) Adenoid lymphocyte responses to outer membrane protein P6 of nontypable Haemophilus influenzae in children with and without otitis media. Acta Otolaryngol Suppl 523:153-4
Faden, H; Duffy, L; Williams, A et al. (1995) Epidemiology of nasopharyngeal colonization with nontypeable Haemophilus influenzae in the first 2 years of life. J Infect Dis 172:132-5
Faden, H; Harabuchi, Y; Hong, J J (1994) Epidemiology of Moraxella catarrhalis in children during the first 2 years of life: relationship to otitis media. J Infect Dis 169:1312-7
Harabuchi, Y; Faden, H; Yamanaka, N et al. (1994) Nasopharyngeal colonization with nontypeable Haemophilus influenzae and recurrent otitis media. Tonawanda/Williamsville Pediatrics. J Infect Dis 170:862-6
Bernstein, J M; Faden, H F; Dryja, D M et al. (1993) Micro-ecology of the nasopharyngeal bacterial flora in otitis-prone and non-otitis-prone children. Acta Otolaryngol 113:88-92
Faden, H; Bernstein, J; Brodsky, L et al. (1992) Effect of prior antibiotic treatment on middle ear disease in children. Ann Otol Rhinol Laryngol 101:87-91
Bernstein, J M; Faden, H S; Loos, B G et al. (1992) Recurrent otitis media with non-typable Haemophilus influenzae: the role of serum bactericidal antibody. Int J Pediatr Otorhinolaryngol 23:1-13
Duffy, L C; Zielezny, M A; Marshall, J R et al. (1991) Relevance of major stress events as an indicator of disease activity prevalence in inflammatory bowel disease. Behav Med 17:101-10
Faden, H; Waz, M J; Bernstein, J M et al. (1991) Nasopharyngeal flora in the first three years of life in normal and otitis-prone children. Ann Otol Rhinol Laryngol 100:612-5

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