The research plan is designed to determine the molecular mechanisms responsible for desensitization of the hormonally-responsive adenylyl cyclase system in the rabbit corpus luteum. This will be accomplished by analyzing the components of luteal adenylyl cyclase upon desensitization to human chorionic gonadotropin (hCG) and catecholamines. The components to be analyzed are the catalytic component, the stimulatory and inhibitory guanine nucleotide binding regulatory components, and receptors for gonadotropins and catecholamines. Through the use of enzyme assays, receptor-binding assays, reconstitution assays, gel electrophoresis, receptor photoaffinity labels and affinity chromatography, the reactions for inducing the desensitized state will be established. Furthermore, we will test the hypothesis that a phosphorylation event is associated with desensitization and determine which components of the luteal adenylyl cyclase system are phosphorylated. Results will shed new light into the mechanisms involved in the regulation of corpus luteum function and should help in the development of contraceptives designed to alter luteal function in early pregnancy or chemotherapeutic agents for gonadal cancers.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD022058-03
Application #
3321342
Study Section
Biochemical Endocrinology Study Section (BCE)
Project Start
1986-08-01
Project End
1988-12-31
Budget Start
1988-08-01
Budget End
1988-12-31
Support Year
3
Fiscal Year
1988
Total Cost
Indirect Cost
Name
Iowa State University
Department
Type
Schools of Arts and Sciences
DUNS #
City
Ames
State
IA
Country
United States
Zip Code
50011