Abstract

The purpose of this program is to test the hypothesis that the pathway, phospholipids to arachidonic acid to prostaglandins is involved in normal and abnormal sexual differentiation. We propose to do this by testing whether arachidonic acid and prostaglandins are able to correct the abnormal development of anogenetal distance induced by agents interfering with the actions of testosterone. We also propose to analyze the release of arachidonic acid, prostaglandin biosynthesis in the presence and absence of agents either including or interfering with masculine differentiation to correlate with the in vivo findings. Moreover, we plan to investigate whether testosterone induces phospholipase A2-stimulating protein (PLAP) in embryonic genitalia and whether PLAP can mimic the action of testosterone.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD022781-04
Application #
3322630
Study Section
Human Embryology and Development Subcommittee 2 (HED)
Project Start
1987-04-01
Project End
1991-03-31
Budget Start
1989-04-01
Budget End
1991-03-31
Support Year
4
Fiscal Year
1989
Total Cost
Indirect Cost

  Institution

Name
Children's Hosp Pittsburgh/Upmc Health Sys
Department
Type
DUNS #
044304145
City
Pittsburgh
State
PA
Country
United States
Zip Code
15224

  Publications

Nguyen, A P; Chandorkar, A; Gupta, C (1996) The role of growth hormone in fetal mouse reproductive tract differentiation. Endocrinology 137:3659-66
Gupta, C (1994) The 72-kilodalton protein of the male reproductive tract is differentially expressed and developmentally regulated during Wolffian duct differentiation of the fetal mouse. Biol Reprod 51:283-9
Gupta, C (1993) Further characterization of the testosterone inducible protein fraction from the mouse fetal male reproductive tract inducing masculine differentiation in vitro. J Steroid Biochem Mol Biol 45:375-81
Gupta, C; Bentlejewski, C (1993) Immunological identification of the protein-producing masculine differentiation of the Wolffian duct in the fetal mouse: western blot analysis and determination of the biological activity. Endocrinology 133:1341-6
Gupta, C; Bentlejewski, C A (1992) Role of prostaglandins in the testosterone-dependent wolffian duct differentiation of the fetal mouse. Biol Reprod 47:1151-60
Gupta, C; Braun, A (1990) Identification and partial purification of embryonic mouse genital protein(s) stimulating phospholipase-A2 and inducing masculinization in vitro. Endocrinology 126:341-8
Gupta, C; Wharton, V; Ellis, D (1990) Anti-masculinizing action of estradiol and cyproterone acetate: regulation of a protein fraction with phospholipase-A2 stimulatory and masculinizing activities. J Steroid Biochem Mol Biol 37:661-7
Gupta, C (1989) Prostaglandins masculinize the mouse genital tract. Endocrinology 124:1781-7
Gupta, C (1989) The role of prostaglandins in masculine differentiation: modulation of prostaglandin levels in the differentiating genital tract of the fetal mouse. Endocrinology 124:129-33
Gupta, C (1988) Activation of phospholipases during masculine differentiation of embryonic genitalia. Proc Soc Exp Biol Med 188:489-94