It now is possible to utilize stable isotopes and gas chromatography-mass spectrometry to perform new kinds of kinetic studies in newborn infants. The objectives of the current proposal are as follows: (1) To use this technology in order to measure the turnover of the secretory proteins albumin and fibronectin in premature newborn infants. This series of investigations involves the administration of the labelled amino acid (15N) glycine to these infants and the subsequent determination of the appearance of this isotope in the albumin and fibronectin. by comparing this rate of appearance with the enrichment of urinary (15N) hippuric acid, which is a marker for intra-hepatic (15N) glycine enrichment, it is possible to measure the fractional synthetic rate (FSR) (day-1) of these secretory proteins. We will ascertain whether the FSR varies with the gestational age and the diet of these infants. The second objective is: (2) To utilize stable isotopes in order to determine whether premature infants are capable of the synthesis of the amino acids glycine and cysteine. This series of studies involves the administration of 13C and 15N precursors of these compounds and the subsequent determination of isotopic abundance in the amino acids of interest. In this way it will be possible to determine in a precise fashion if these amino acids are essential for premature infants. The synthesis of cysteine and glycine will be correlated with the gestational age of the infant. Kinetic studies such as these are of importance in establishing the nutritional needs of premature infants.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD023168-03
Application #
3323210
Study Section
Nutrition Study Section (NTN)
Project Start
1987-09-01
Project End
1990-12-31
Budget Start
1989-09-01
Budget End
1990-12-31
Support Year
3
Fiscal Year
1989
Total Cost
Indirect Cost
Name
Children's Hospital of Philadelphia
Department
Type
DUNS #
073757627
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
Lu, Yani; Ma, Huiyan; Malone, Kathleen E et al. (2011) Obesity and survival among black women and white women 35 to 64 years of age at diagnosis with invasive breast cancer. J Clin Oncol 29:3358-65
Lu, Yani; Ma, Huiyan; Malone, Kathleen E et al. (2011) Oral contraceptive use and survival in women with invasive breast cancer. Cancer Epidemiol Biomarkers Prev 20:1391-7
Press, David J; Sullivan-Halley, Jane; Ursin, Giske et al. (2011) Breast cancer risk and ovariectomy, hysterectomy, and tubal sterilization in the women's contraceptive and reproductive experiences study. Am J Epidemiol 173:38-47
Li, Christopher I; Mathes, Robert W; Malone, Kathleen E et al. (2009) Relationship between migraine history and breast cancer risk among premenopausal and postmenopausal women. Cancer Epidemiol Biomarkers Prev 18:2030-4
Li, Christopher I; Malone, Kathleen E; Daling, Janet R et al. (2008) Timing of menarche and first full-term birth in relation to breast cancer risk. Am J Epidemiol 167:230-9
Gail, Mitchell H; Costantino, Joseph P; Pee, David et al. (2007) Projecting individualized absolute invasive breast cancer risk in African American women. J Natl Cancer Inst 99:1782-92
Li, Christopher I; Daling, Janet R; Malone, Kathleen E et al. (2006) Relationship between established breast cancer risk factors and risk of seven different histologic types of invasive breast cancer. Cancer Epidemiol Biomarkers Prev 15:946-54
Yudkoff, M; Nissim, I; Hertz, L (1990) Precursors of glutamic acid nitrogen in primary neuronal cultures: studies with 15N. Neurochem Res 15:1191-6
Yudkoff, M; Pleasure, D; Cregar, L et al. (1990) Glutathione turnover in cultured astrocytes: studies with [15N]glutamate. J Neurochem 55:137-45
Schiffer, S P; Prochazka, M; Jezyk, P F et al. (1989) Organic aciduria and butyryl CoA dehydrogenase deficiency in BALB/cByJ mice. Biochem Genet 27:47-58