Endometriosis is one of the most enigmatic and problematic maladies affecting reproductive age women. When male factors are excluded, endometriosis represents the most common cause of reproductive failure in women. Recent evidence suggests that immunologic mechanisms may play a role in the pathogenesis of endometriosis. Immune mechanisms may modulate the development of endometriotic implants and may contribute to the high rate of infertility and spontaneous abortion associated with endometriosis. The objective of this proposal is to investigate the role of cell mediated immunity (CMI) in reproductive failure in women with endometriosis. The incidence of local CMI reactions in the peritoneal fluid of women with endometriosis will be determined by immunohistologic analysis of lymphocytes and macrophage populations and by measurement of levels of lymphokines and monokines in the peritoneal fluid of these women. To perform these studies, the immunoperoxidase technique for detection of characteristic leukocyte markers, and enzyme linked immunosorbent (ELISA) assays for detection of lymphokines and monokines, will be adapted for use with peritoneal fluid. Presence, numbers and concentrations of lymphocyte and macrophage subpopulations and levels of specific lymphokines and monokines from the peritoneal fluid and peripheral blood controls will be correlated with the presence or absence of endometriosis, the stage of the disease and reproductive history. Furthermore, we will continue our basic studies on the effects of soluble products of peritoneal derived leukocytes on reproductive cells and reproductive functions in vitro. These studies may provide insight into new immunological mechanisms underlying reproductive failure in women with endometriosis and new approaches to more effectively diagnose and treat endometriosis- associated reproductive failure.

Project Start
1988-02-01
Project End
1991-01-31
Budget Start
1988-02-01
Budget End
1989-01-31
Support Year
1
Fiscal Year
1988
Total Cost
Indirect Cost
Name
Brigham and Women's Hospital
Department
Type
DUNS #
071723621
City
Boston
State
MA
Country
United States
Zip Code
02115
D'Hooghe, T M; Bambra, C S; Xiao, L et al. (2001) Effect of menstruation and intrapelvic injection of endometrium on inflammatory parameters of peritoneal fluid in the baboon (Papio anubis and Papio cynocephalus). Am J Obstet Gynecol 184:917-25
D'Hooghe, T M; Pudney, J; Hill, J A (2001) Immunobiology of the reproductive tract in a female baboon. Am J Primatol 53:47-54
D'Hooghe, T M; Xiao, L; Hill, J A (2001) Cytokine profiles in autologous peritoneal fluid and peripheral blood of women with deep and superficial endometriosis. Arch Gynecol Obstet 265:40-4
D'Hooghe, T M; Bambra, C S; Raeymaekers, B M et al. (1999) Pelvic inflammation induced by diagnostic laparoscopy in baboons. Fertil Steril 72:1134-41
Marqusee, E; Hill, J A; Mandel, S J (1997) Thyroiditis after pregnancy loss. J Clin Endocrinol Metab 82:2455-7
Hill, J A (1997) Immunology and endometriosis. Fact, artifact, or epiphenomenon? Obstet Gynecol Clin North Am 24:291-306
Chiang, C M; Hill, J A (1997) Localization of T cells, interferon-gamma and HLA-DR in eutopic and ectopic human endometrium. Gynecol Obstet Invest 43:245-50
Bermas, B L; Hill, J A (1997) Proliferative responses to recall antigens are associated with pregnancy outcome in women with a history of recurrent spontaneous abortion. J Clin Invest 100:1330-4
Polgar, K; Yacono, P W; Golan, D E et al. (1996) Immune interferon gamma inhibits translational mobility of a plasma membrane protein in preimplantation stage mouse embryos: a T-helper 1 mechanism for immunologic reproductive failure. Am J Obstet Gynecol 174:282-7
Best, C L; Pudney, J; Welch, W R et al. (1996) Localization and characterization of white blood cell populations within the human ovary throughout the menstrual cycle and menopause. Hum Reprod 11:790-7

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