This study is directed towards examining the onset and control, during development, of glucocortocoid receptor inducible regulatory systems. We plan to examine the developmental generation of this system using early mouse stem cell systems and also the mouse embryo. Our starting point is the mouse embryonal carcinoma cell (or EC cell) since we and others have shown the absence of a glucocortocoid system in these stem cells. We plan to examine the basis(es) for the absence of a glucocortocoid response in mouse embryonal carcinoma cells through examination of glucocortocoid receptor levels, the expression and nature of glucocortocoid receptor mRNA levels, and through introducing vectors for expression of the glucocortocoid receptor. Using stem cell lines and mouse embryos, we plan to examine the developmental timing of the onset of the glucocortocoid response, its possible role in early differentiation and whether we might interfere with this role through gene transfer experiments.
| Smith, B R; Johnson, G A; Groman, E V et al. (1994) Magnetic resonance microscopy of mouse embryos. Proc Natl Acad Sci U S A 91:3530-3 |
| Linney, E (1992) Retinoic acid receptors: transcription factors modulating gene regulation, development, and differentiation. Curr Top Dev Biol 27:309-50 |
| Balkan, W; Klintworth, G K; Bock, C B et al. (1992) Transgenic mice expressing a constitutively active retinoic acid receptor in the lens exhibit ocular defects. Dev Biol 151:622-5 |
| Espeseth, A S; Murphy, S P; Linney, E (1989) Retinoic acid receptor expression vector inhibits differentiation of F9 embryonal carcinoma cells. Genes Dev 3:1647-56 |
