Abstract

The mRNA for tissue plasminogen activator (t-PA) is present in primary mouse ooctyes but not translated. Upon meiotic maturation, this mRNA has 400-500 (A) residues added to the 3' end, begins to be translated, and is degraded. Using this system, and approaches developed to study t-PA mRNA recruitment, we propose the following: 1. To investigate using injection of RNA fragments and chimeric mRNAs the sequences in t-PA mRNA that determine its precise elongation, translational activation and subsequent degradation during oocyte maturation. 2. To search for molecules involved in oocyte maturation, meiosis and fertilization using cDNA libraries prepared from primary oocytes. 3. To assess whether information about meiosis gathered from other species, notably the fission yeast Schizosaccharomyces pombe and Xenopus laevis, can be used to further understand this process in mammals.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
1R01HD025922-01
Application #
3327170
Study Section
Reproductive Biology Study Section (REB)
Project Start
1989-09-01
Project End
1993-05-31
Budget Start
1989-09-01
Budget End
1990-05-31
Support Year
1
Fiscal Year
1989
Total Cost
Indirect Cost

  Institution

Name
State University New York Stony Brook
Department
Type
Schools of Medicine
DUNS #
804878247
City
Stony Brook
State
NY
Country
United States
Zip Code
11794

  Publications

Salles, F J; Strickland, S (1999) Analysis of poly(A) tail lengths by PCR: the PAT assay. Methods Mol Biol 118:441-8
Verrotti, A C; Strickland, S (1997) Oocyte selection of mutations affecting cytoplasmic polyadenylation of maternal mRNAs. Mol Reprod Dev 46:482-8
Wells, J M; Strickland, S (1997) Regulated localization confers multiple functions on the protease urokinase plasminogen activator. J Cell Physiol 171:217-25
West, M F; Verrotti, A C; Salles, F J et al. (1996) Isolation and characterization of two novel, cytoplasmically polyadenylated, oocyte-specific, mouse maternal RNAs. Dev Biol 175:132-41
Verrotti, A C; Thompson, S R; Wreden, C et al. (1996) Evolutionary conservation of sequence elements controlling cytoplasmic polyadenylylation. Proc Natl Acad Sci U S A 93:9027-32
Salles, F J; Strickland, S (1995) Rapid and sensitive analysis of mRNA polyadenylation states by PCR. PCR Methods Appl 4:317-21
Wells, J M; Strickland, S (1994) Aprotinin, a Kunitz-type protease inhibitor, stimulates skeletal muscle differentiation. Development 120:3639-47
Carroll, P M; Richards, W G; Darrow, A L et al. (1993) Preimplantation mouse embryos express a cell surface receptor for tissue-plasminogen activator. Development 119:191-8
Huarte, J; Stutz, A; O'Connell, M L et al. (1992) Transient translational silencing by reversible mRNA deadenylation. Cell 69:1021-30
Salles, F J; Darrow, A L; O'Connell, M L et al. (1992) Isolation of novel murine maternal mRNAs regulated by cytoplasmic polyadenylation. Genes Dev 6:1202-12

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