Recent finding from this laboratory suggested that EGF plays a role during androgen-induced fetal reproductive tract development. We propose to investigate its role further by testing: 1. whether Western blot positive different EGF-immunoactive protein molecules present in the fetal male reproductive tract, are modulated in response to testosterone-induced male reproductive tract differentiation; 2. whether EGF-mRNA synthesis is induced in response to this differentiation and whether testosterone plays a role in the induction ; 3. whether in situ hybridization of the developing reproductive duct detects a cell type producing increased expression of EGF-mRNA in response to this differentiation; 4. whether EGF receptor plays a role by determining the effect of the anti-EGF receptor antibody in organ culture assay of the differentiation and by determining its expression using Western blot and immunohistological analysis during the Wolffian duct differentiation; 5. whether EGF receptor-mRNA synthesis is modulated during the male reproductive differentiation and whether testosterone plays a role in this induction.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD027974-03
Application #
2200796
Study Section
Reproductive Endocrinology Study Section (REN)
Project Start
1994-07-01
Project End
1999-06-30
Budget Start
1996-07-01
Budget End
1999-06-30
Support Year
3
Fiscal Year
1996
Total Cost
Indirect Cost
Name
Children's Hosp Pittsburgh/Upmc Health Sys
Department
Type
DUNS #
044304145
City
Pittsburgh
State
PA
Country
United States
Zip Code
15224