Dr. Hortsch proposes to continue his investigation of the mechanism by which neuroglian/L1-CAM recruits ankyrin to the vicinity of the plasma membrane in Drosophila embryos and transfected S2 cells. He proposes a number of straightforward deletion and replacement mutagenesis studies to analyze which domains of neuroglian are required to recruit ankyrin. He also will use yeast two-hybrid and ankyrin affinity chromatography approaches to identify additional ankyrin-binding membrane proteins in Drosophila and will use transgenic Drosophila lines containing myc-tagged ankyrin to demonstrate the in vivo effects of neuroglian mutants. Finally, he will use this assay system to test whether mutant L1-CAM molecules from human patients will function in his assay.
| Goossens, Tim; Kang, Yuan Y; Wuytens, Gunther et al. (2011) The Drosophila L1CAM homolog Neuroglian signals through distinct pathways to control different aspects of mushroom body axon development. Development 138:1595-605 |
| Carhan, A; Allen, F; Armstrong, J D et al. (2005) Female receptivity phenotype of icebox mutants caused by a mutation in the L1-type cell adhesion molecule neuroglian. Genes Brain Behav 4:449-65 |
| Islam, Rafique; Kristiansen, Lars V; Romani, Susana et al. (2004) Activation of EGF receptor kinase by L1-mediated homophilic cell interactions. Mol Biol Cell 15:2003-12 |
| Wei, Jun; Hortsch, Michael; Goode, Scott (2004) Neuroglian stabilizes epithelial structure during Drosophila oogenesis. Dev Dyn 230:800-8 |
| Malhotra, Jyoti D; Koopmann, Matthew C; Kazen-Gillespie, Kristin A et al. (2002) Structural requirements for interaction of sodium channel beta 1 subunits with ankyrin. J Biol Chem 277:26681-8 |
