Abstract

The goal of this proposal is to define the in vivo role of trophinin in embryo implantation using the mouse as a model.
The specific aims are: 1) to define the in vivo role of trophinin by analyzing trophinin gene knockout mice; 2) to define the expression and detailed localization of trophinin in mouse blastocysts and endometrium by determining the localization of trophinin in mouse blastocyst and endometrial epithelium at peri-implantation periods by immunoelectron microscopy, 3) to define the interaction between trophinin and tastin by yeast two-hybrid assays, and 4) to define the promoter activity of the human trophinin gene in order to understand the temporal and spatial expression of the trophinin in the cells involved in implantation.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD034108-04
Application #
6138794
Study Section
Human Embryology and Development Subcommittee 1 (HED)
Program Officer
Yoshinaga, Koji
Project Start
1997-01-01
Project End
2001-12-31
Budget Start
2000-01-01
Budget End
2000-12-31
Support Year
4
Fiscal Year
2000
Total Cost
$301,355
Indirect Cost

  Institution

Name
Sanford-Burnham Medical Research Institute
Department
Type
DUNS #
020520466
City
La Jolla
State
CA
Country
United States
Zip Code
92037

  Publications

Sugihara, K; Kobayashi, Y; Suzuki, A et al. (2014) Development of pro-apoptotic peptides as potential therapy for peritoneal endometriosis. Nat Commun 5:4478
Sugihara, Kazuhiro; Kabir-Salmani, Maryam; Byrne, James et al. (2008) Induction of trophinin in human endometrial surface epithelia by CGbeta and IL-1beta. FEBS Lett 582:197-202
Harada, Oi; Suga, Tomoaki; Suzuki, Takefumi et al. (2007) The role of trophinin, an adhesion molecule unique to human trophoblasts, in progression of colorectal cancer. Int J Cancer 121:1072-8
Sugihara, Kazuhiro; Sugiyama, Daijiro; Byrne, James et al. (2007) Trophoblast cell activation by trophinin ligation is implicated in human embryo implantation. Proc Natl Acad Sci U S A 104:3799-804
Aoki, Rui; Suzuki, Nao; Paria, Bibhash C et al. (2006) The Bysl gene product, bystin, is essential for survival of mouse embryos. FEBS Lett 580:6062-8
Sheng, Jiansong; Yang, Shuo; Xu, Lei et al. (2004) Bystin as a novel marker for reactive astrocytes in the adult rat brain following injury. Eur J Neurosci 20:873-84
Hatakeyama, Shingo; Ohyama, Chikara; Minagawa, Shingo et al. (2004) Functional correlation of trophinin expression with the malignancy of testicular germ cell tumor. Cancer Res 64:4257-62
Nozawa, Shiro; Aoki, Daisuke; Tsukazaki, Katsumi et al. (2004) HMMC-1: a humanized monoclonal antibody with therapeutic potential against Mullerian duct-related carcinomas. Clin Cancer Res 10:7071-8
Nakayama, Jun; Aoki, Daisuke; Suga, Tomoaki et al. (2003) Implantation-dependent expression of trophinin by maternal fallopian tube epithelia during tubal pregnancies: possible role of human chorionic gonadotrophin on ectopic pregnancy. Am J Pathol 163:2211-9
Nadano, Daita; Nakayama, Jun; Matsuzawa, Shu-Ichi et al. (2002) Human tastin, a proline-rich cytoplasmic protein, associates with the microtubular cytoskeleton. Biochem J 364:669-77

Showing the most recent 10 out of 17 publications