This application will try to achieve the long term culture of type A spermatogonia.
Three specific aims are proposed: 1) to determine the basement membrane components that are optimal for allowing long-term (longer than 1 week) survival of type A spermatogonia in culture; 2) to test the hypothesis that there is synergism between growth factors and basement membrane components in promoting long-term survival of spermatogonia; and 3) to develop immortalized cell lines of type A spermatogonia that will propagate in culture without losing their original properties.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD036483-04
Application #
6387957
Study Section
Special Emphasis Panel (ZHD1-MRG-C (04))
Program Officer
Tasca, Richard J
Project Start
1998-04-01
Project End
2003-03-31
Budget Start
2001-04-01
Budget End
2002-03-31
Support Year
4
Fiscal Year
2001
Total Cost
$227,188
Indirect Cost
Name
Georgetown University
Department
Biochemistry
Type
Schools of Medicine
DUNS #
049515844
City
Washington
State
DC
Country
United States
Zip Code
20057
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