This research is directed to an important problem in ovarian biology, the underlying mechanisms through which the corpus luteum is disposed of after each estrous cycle. The P. I. has made the important observation that the steroidogenic luteal cells of the cow express class II major histocompatibility molecules, a property normally found only on professional antigen presenting immune/inflammatory cells. This unusual property of luteal cells and other observations have led the P. I. to the interesting and provocative hypothesis, that the luteal cells are the participants in a transient autoimmune response, which leads to rejection of the luteal tissue at the end of each cycle. Thus the Aims are: 1) characterize the expression of elements necessary for antigen presentation associated with class I and class II major histocompatibility molecules; 2) investigate the regulation of expression of specific proteins that comprise the antigen processing complex; 3) investigate the expression, functional significance and regulation of co-stimulatory molecules; 4) determine whether engagement between luteal cells and T lymphocytes is mediated through MHC class I and /or MHC class II-restricted interaction; 5) determine if alterations occur in the peptides presented by MHC class I and class II molecules expressed by functional vs. regressing corpora lutea. These studies are expected to reveal whether luteal cells have the requisite characteristics of antigen presenting cells, thus providing new information as test of a proposed transient autoimmune reaction during regression of the corpus luteum.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD037550-05
Application #
6847468
Study Section
Reproductive Biology Study Section (REB)
Program Officer
Taymans, Susan
Project Start
2001-03-01
Project End
2008-02-28
Budget Start
2005-03-01
Budget End
2008-02-28
Support Year
5
Fiscal Year
2005
Total Cost
$165,938
Indirect Cost
Name
Ohio State University
Department
Veterinary Sciences
Type
Schools of Earth Sciences/Natur
DUNS #
832127323
City
Columbus
State
OH
Country
United States
Zip Code
43210
Ndiaye, Kalidou; Lussier, Jacques G; Pate, Joy L (2010) Molecular characterization and expression of DERL1 in bovine ovarian follicles and corpora lutea. Reprod Biol Endocrinol 8:94
Davis, Tracy L; Pate, Joy L (2007) Bovine luteal cells stimulate proliferation of major histocompatibility nonrestricted gamma delta T cells. Biol Reprod 77:914-22
Cannon, Matthew J; Davis, John S; Pate, Joy L (2007) The class II major histocompatibility complex molecule BoLA-DR is expressed by endothelial cells of the bovine corpus luteum. Reproduction 133:991-1003
Cannon, Matthew J; Davis, John S; Pate, Joy L (2007) Expression of costimulatory molecules in the bovine corpus luteum. Reprod Biol Endocrinol 5:5
Cannon, Matthew J; Pate, Joy L (2006) Indoleamine 2,3-dioxygenase participates in the interferon-gamma-induced cell death process in cultured bovine luteal cells. Biol Reprod 74:552-9
Cannon, Matthew J; Davis, John S; Pate, Joy L (2006) Presence and regulation of messenger ribonucleic acids encoding components of the class II major histocompatibility complex-associated antigen processing pathway in the bovine corpus luteum. Reproduction 131:689-98
Cannon, Matthew J; Pate, Joy L (2003) The role of major histocompatibility complex molecules in luteal function. Reprod Biol Endocrinol 1:93
Cannon, Matthew J; Petroff, Margaret G; Pate, Joy L (2003) Effects of prostaglandin F2alpha and progesterone on the ability of bovine luteal cells to stimulate T lymphocyte proliferation. Biol Reprod 69:695-700
Cannon, Matthew J; Pate, Joy L (2003) Expression and regulation of interferon gamma-inducible proteasomal subunits LMP7 and LMP10 in the bovine corpus luteum. Biol Reprod 68:1447-54