The diaphragm is an essential mammalian skeletal muscle, as it is vital for respiration and serves as a barrier between the thoracic and abdominal cavities. Development of the diaphragm requires the integration of multiple tissues that derive from several embryonic sources. Defects in diaphragm development are the cause of congenital diaphragmatic hernias (CDHs), a common birth defect (1:3000 births) that results in severe morbidity and 50% mortality. Given the diaphragm's functional importance and the frequency and severity of CDH, an understanding of diaphragm development normally and during herniation is critical. Recently, using mouse genetics, we definitively established that the pleuroperitoneal folds, transient embryonic structures, and the muscle connective tissue fibroblasts derived from them critically regulate development of the diaphragm muscle (Merrell et al. 2015). Furthermore, we showed that mutations in these fibroblasts cause CDH. However, the molecular signals from the fibroblasts that regulate muscle development normally and are defective in CDH are not yet known. Based on preliminary studies, we hypothesize that connective tissue fibroblasts are an important source of secreted signals that recruit muscle progenitors into the developing diaphragm; regulate muscle morphogenesis; and are mis-regulated in CDH. In addition, our mouse studies (Merrell et al. 2015) suggest the novel hypothesis that somatic mosaic mutations in connective tissue fibroblasts are critical for the etiology of CDH ? a hypothesis that may explain the genetic complexity and phenotypic variability of CDH. We propose to use mouse genetic studies and CDH patient samples to test these hypotheses. Our research will elucidate the genetic, molecular, and cellular mechanisms regulating the development of the diaphragm and CDH and provide important insights into potential therapeutic targets to treat CDH.

Public Health Relevance

The diaphragm is an essential mammalian skeletal muscle, as it is vital for respiration and serves as a barrier between the thoracic and abdominal cavities. Development of the diaphragm requires the integration of multiple tissues that derive from several embryonic sources, and defects in diaphragm development are the cause of congenital diaphragmatic hernia (CDH) a common birth defect (1 in 3000 births) that results in severe morbidity and 50% mortality. Our research will elucidate the genetic, molecular, and cellular mechanisms regulating development of the diaphragm and CDH and provide important insights into potential therapeutic targets to treat CDH.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
1R01HD087360-01A1
Application #
9185184
Study Section
Skeletal Muscle Biology and Exercise Physiology Study Section (SMEP)
Program Officer
Javois, Lorette Claire
Project Start
2016-08-01
Project End
2021-04-30
Budget Start
2016-08-01
Budget End
2017-04-30
Support Year
1
Fiscal Year
2016
Total Cost
Indirect Cost
Name
University of Utah
Department
Genetics
Type
Schools of Medicine
DUNS #
009095365
City
Salt Lake City
State
UT
Country
United States
Zip Code
84112
Sefton, Elizabeth M; Gallardo, Mirialys; Kardon, Gabrielle (2018) Developmental origin and morphogenesis of the diaphragm, an essential mammalian muscle. Dev Biol 440:64-73
Kardon, Gabrielle; Ackerman, Kate G; McCulley, David J et al. (2017) Congenital diaphragmatic hernias: from genes to mechanisms to therapies. Dis Model Mech 10:955-970
Wan, Yong; Otsuna, Hideo; Holman, Holly A et al. (2017) FluoRender: joint freehand segmentation and visualization for many-channel fluorescence data analysis. BMC Bioinformatics 18:280