We propose to develop a novel, cost-efficient, high-throughput multiplex technology, based on time-of-flight mass spectrometry as the analysis tool, which can be broadly applied in the area of genomic analysis. We intend to demonstrate and validate the utility of this technology in four areas: gene expression, gene discovery, mRNA sizing and polymorphism detection. We will develop a high-throughput, solution-phase assay for monitoring mRNA expression levels. We will develop a highly parallelized method for identifying all cDNAs within a given cDNA library and for fast identification of novel cDNAs. We will develop a multiplexed approach to accurately sizing individual mRNAs. And we will develop a high throughput and cost effective multiplexed assay for the detection of single nucleotide polymorphisms.

Agency
National Institute of Health (NIH)
Institute
National Human Genome Research Institute (NHGRI)
Type
Research Project (R01)
Project #
5R01HG001700-03
Application #
2889681
Study Section
Special Emphasis Panel (ZHG1-HGR-N (O1))
Program Officer
Graham, Bettie
Project Start
1997-09-30
Project End
2001-05-31
Budget Start
1999-09-01
Budget End
2001-05-31
Support Year
3
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Genetrace Systems, Inc.
Department
Type
DUNS #
City
Alameda
State
CA
Country
United States
Zip Code
94502