Abstract

This partnership will bridge the technology development efforts of Caltech faculty in Applied Physics and Bioengineering with clinical needs of faculty at the USC Keck School of Medicine. We will apply technology for nanofluidic chips that has been developed at Caltech to problems of biological and medical interest. Using the combined resources of the Applied Physics and the Bioengineering programs, we will develop new nanotechnology to solve a number of bioengineering obstacles that presently exist for single cell genomic analysis on a chip. We will develop microfabricated chips with the ability to manipulate nanoliters of fluid; these chips will be used to perform highly parallel biochemical manipulations and genetic analyses of rare populations of cells. The chips will be able to create unique reagents that can be analyzed using conventional functional genomics techniques. A chip foundry will be created in order to produce research quantities of these chips that can be shared among collaborators. The chip technology will be used to investigate the following two problems of particular medical importance: factor and marker discovery in haematopoietic stem cells, and the discovery and characterization of unculturable pathogens in the human gut.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Human Genome Research Institute (NHGRI)
Type
Research Project (R01)
Project #
1R01HG002644-01A1
Application #
6701416
Study Section
Special Emphasis Panel (ZRG1-GNM (90))
Program Officer
Schloss, Jeffery
Project Start
2004-06-05
Project End
2009-04-30
Budget Start
2004-06-05
Budget End
2005-04-30
Support Year
1
Fiscal Year
2004
Total Cost
$1,000,000
Indirect Cost

  Institution

Name
California Institute of Technology
Department
Type
Schools of Engineering
DUNS #
009584210
City
Pasadena
State
CA
Country
United States
Zip Code
91125

  Publications

Ottesen, Elizabeth A; Leadbetter, Jared R (2011) Formyltetrahydrofolate synthetase gene diversity in the guts of higher termites with different diets and lifestyles. Appl Environ Microbiol 77:3461-7
Maltezos, George; Lee, John; Rajagopal, Aditya et al. (2011) Microfluidic blood filtration device. Biomed Microdevices 13:143-6
Ottesen, Elizabeth A; Leadbetter, Jared R (2010) Diversity of formyltetrahydrofolate synthetases in the guts of the wood-feeding cockroach Cryptocercus punctulatus and the omnivorous cockroach Periplaneta americana. Appl Environ Microbiol 76:4909-13
Liu, John; Chen, Yan; Taylor, Clive R et al. (2009) Elastomeric microfluidic diode and rectifier work with Newtonian fluids. J Appl Phys 106:114311
Fan, H Christina; Blumenfeld, Yair J; El-Sayed, Yasser Y et al. (2009) Microfluidic digital PCR enables rapid prenatal diagnosis of fetal aneuploidy. Am J Obstet Gynecol 200:543.e1-7
Gerber, Doron; Maerkl, Sebastian J; Quake, Stephen R (2009) An in vitro microfluidic approach to generating protein-interaction networks. Nat Methods 6:71-4
Kartalov, Emil P; Lin, David H; Lee, David T et al. (2008) Internally calibrated quantification of protein analytes in human serum by fluorescence immunoassays in disposable elastomeric microfluidic devices. Electrophoresis 29:5010-6
Einav, Shirit; Gerber, Doron; Bryson, Paul D et al. (2008) Discovery of a hepatitis C target and its pharmacological inhibitors by microfluidic affinity analysis. Nat Biotechnol 26:1019-27
Kartalov, Emil P; Maltezos, George; Anderson, W French et al. (2007) Electrical microfluidic pressure gauge for elastomer microelectromechanical systems. J Appl Phys 102:84909-849094
Zhong, Jiang F; Song, Yahui; Du, Jing et al. (2007) Gene regulation networks related to neural differentiation of hESC. Gene Expr 14:23-34

Showing the most recent 10 out of 22 publications