Significant enhancements in gene sequencing may be achieved through implementation of analysis instruments at the same dimensional scale as DNA, i.e nanometers. Nanotechnology has recently provided the necessary tools to create such nanoinstruments and this proposal seeks to utilize these tools to fabricate a high-speed, low-cost gene sequencer. The gene sequencer is based on the nanopore approach and incorporates tunneling current electrodes to sense the individual nucleotides as they transverse the pore. ? ? Specific aims for this proposal are: ? 1). Design and fabricate nanopore devices complete with tunneling current electrodes and integrated sense and control circuitry on a chip. ? 2). Characterize the nanopore device using known ssDNA sequences. Initial characterization will be limited to DNA strands of approximately 1000 bases. Subsequent evaluations will include selected bacteria, and mammalian (mouse) DNA. ? ?

Agency
National Institute of Health (NIH)
Institute
National Human Genome Research Institute (NHGRI)
Type
Research Project (R01)
Project #
5R01HG003565-02
Application #
6953253
Study Section
Special Emphasis Panel (ZHG1-HGR-N (O1))
Program Officer
Schloss, Jeffery
Project Start
2004-09-29
Project End
2006-09-30
Budget Start
2005-07-01
Budget End
2006-04-30
Support Year
2
Fiscal Year
2005
Total Cost
$359,195
Indirect Cost
Name
University of Maine Orono
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
186875787
City
Orono
State
ME
Country
United States
Zip Code
04469
Chen, Shiahn J; Howitt, David G; Gierhart, Brian C et al. (2009) The applications of in situ electron energy loss spectroscopy to the study of electron beam nanofabrication. Microsc Microanal 15:204-12
Gierhart, Brian C; Howitt, David G; Chen, Shiahn J et al. (2008) Nanopore with Transverse Nanoelectrodes for Electrical Characterization and Sequencing of DNA. Sens Actuators B Chem 132:593-600
Gierhart, Brian C; Howitt, David G; Chen, Shiahn J et al. (2007) Frequency dependence of gold nanoparticle superassembly by dielectrophoresis. Langmuir 23:12450-6