Long-term objectives: analysis of the nature and ionic requirements of the mechanisms that regulate intracellular pH (pHi).
Specific aims : (1) investigation of the mechanism by which 1- methyl, 3-isobutylxanthine (IBMX), an inhibitor of cyclic nucleotide phosphodiesterase, stimulates, in frog skeletal muscle, recovery of pHi from imposed acidification; (2) kinetic analysis of the mechanism by which the transporter in giant barnacle muscle removes acid equivalents from the fiber in response to acidification. General methods: electrophysiological methods, intracellular pH- sensitive microelectrodes.
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| Amorena, C E; Wilding, T J; Manchester, J K et al. (1990) Changes in intracellular pH caused by high K in normal and acidified frog muscle. Relation to metabolic changes. J Gen Physiol 96:959-72 |
| Putnam, R W; Roos, A; Wilding, T J (1986) Properties of the intracellular pH-regulating systems of frog skeletal muscle. J Physiol 381:205-19 |
| Putnam, R W; Roos, A (1986) Effect of calcium and other divalent cations on intracellular pH regulation of frog skeletal muscle. J Physiol 381:221-39 |
