Studies on Cytoskeletal Proteins in Platelets

Stracher, Alfred

Abstract

These studies are concerned with the isolation and characterization of the proteins associated with the cytoskeletal structure of human blood platelets. Two proteins, F-actin and actin-binding protein (ABP) have been isolated from platelets by a simplified procedure and when these are reassociated they form a structure resembling that which exists in the native platelet. Studies ar in progress to characterize the factors which may be involved in regulating the assembly-disassembly of the cytoskeleton of platelets.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Heart, Lung, and Blood Institute (NHLBI)
• Type: Research Project (R01)
• Project #: 5R01HL014020-12
• Application #: 3334716
• Study Section: (EH)
• Project Start: 1978-12-01
• Project End: 1986-11-30
• Budget Start: 1984-12-01
• Budget End: 1985-11-30
• Support Year: 12
• Fiscal Year: 1985

Institution

• Name: Suny Downstate Medical Center
• Type: Schools of Medicine
• DUNS #: 068552207
• City: Brooklyn
• State: NY
• Country: United States
• Zip Code: 11203

Publications

Qingqi, Z; Stracher, A (1998) Platelet membrane actin may be partially embedded in lipid bilayer and disulfide linked. Biochem Biophys Res Commun 252:407-11

Jay, D; Stracher, A (1997) Expression in Escherichia coli, phosphorylation with cAMP-dependent protein kinase and proteolysis by calpain of a 71-kDa domain of human endothelial actin binding protein. Biochem Biophys Res Commun 232:555-8

Wu, M P; Jay, D; Stracher, A (1994) Existence of multiple phosphorylated forms of human platelet actin binding protein. Cell Mol Biol Res 40:351-7

Sterling, H; Stracher, A (1993) Platelet membrane-actin interaction. Cell Mol Biol Res 39:53-63

Shu, W P; Wang, D; Stracher, A (1992) Chemical evidence for the existence of activated G-actin. Biochem J 283 ( Pt 2):567-73

Liu, D F; Wang, D; Stracher, A (1990) The accessibility of the thiol groups on G- and F-actin of rabbit muscle. Biochem J 266:453-9

Chen, M; Stracher, A (1989) In situ phosphorylation of platelet actin-binding protein by cAMP-dependent protein kinase stabilizes it against proteolysis by calpain. J Biol Chem 264:14282-9

Zhuang, Q; Stracher, A (1989) Purification and characterization of a calcium binding protein with ""synexin-like"" activity from human blood platelets. Biochem Biophys Res Commun 159:236-41

Zhang, Z; Lawrence, J; Stracher, A (1988) Phosphorylation of platelet actin binding protein protects against proteolysis by calcium dependent sulfhydryl protease. Biochem Biophys Res Commun 151:355-60