lnterrelated functions of hemostatic, kinin-releasing, and fibrinolytic enzymes in plasma and the serum complement system will be examined to gain insight into the ways in which some of those enzymes are regulated by the serum C1-inhibitor, which regulates several such enzymes but is critical to the regulation of C1, the activated first component of complement. In addition, hemostatic events involving high molecular weight kininogen may affect vascular function, and the interactions between this kininogen and endothelium will be examined. To do this, several approaches will be used: 1. Purified normal C1-inhibitor will be examined to determine if different domains of the molecule may participate in site-specific interactions with the plasma proteases known to be regulated by this inhibitor. This possibility is likely because our earlier studies showed heterogeneous behavior of abnormal C1-inhibitor proteins from persons with hereditary angioneurotic edema with respect to each of the enzymes regulated by normal C1-inhibitor. To do this, fragments of normal C1-inhibitor generated with cyanogen bromide, or by limited proteolytic digestion, will be examined for their inhibitory activity and their ability to form complexes-with these proteases (factor Xlla, kallikrein, plasmin, C1s. 2. There are multiple possible explanations for altered expression of the C1-inhibitor gene in hereditary angioneurotic edema. One approach to be used in this laboratory to gain insights into this problem will be to compare mRNA from peripheral blood monocytes of normal persons to that from persons with different types of hereditary angioneurotic edema. Both the quantity and quality of mRNA from these patients will be compared to that from normal individuals. 3. Cultured human umbilical endothelial cells will be tested for antigens located on the heavy or light chains of human high molecular weight kininogen. Since light chain participates in binding to certain substances and in generating clot-promoting activity, it might be identified on endothelial membranes, and might transfer to these cells under definable states in which its function would be provoked. Additional studies will examine the ratios of plasma concentrations of light and heavy chain antigens of HMWK in plasma from normal persons- and those with pathologic states involving the vasculature.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL015690-19
Application #
3335022
Study Section
Hematology Subcommittee 2 (HEM)
Project Start
1978-09-01
Project End
1994-03-31
Budget Start
1991-04-01
Budget End
1992-03-31
Support Year
19
Fiscal Year
1991
Total Cost
Indirect Cost
Name
Cincinnati Children's Hospital Medical Center
Department
Type
DUNS #
071284913
City
Cincinnati
State
OH
Country
United States
Zip Code
45229
Donaldson, V H; Wagner, C J; Mitchell, B H et al. (1998) An HMG-I protein from human endothelial cells apparently is secreted and impairs activation of Hageman factor (factor XII). Proc Assoc Am Physicians 110:140-9
Donaldson, V H; Wagner, C J; Davis 3rd, A E (1996) An autoantibody to C1-inhibitor recognizes the reactive center of the inhibitor. J Lab Clin Med 127:229-32
Donaldson, V H; Bissler, J J; Welch, T R et al. (1996) Antibody to C1-inhibitor in a patient receiving C1-inhibitor infusions for treatment of hereditary angioneurotic edema with systemic lupus erythematosus reacts with a normal allotype of residue 458 of C1-inhibitor. J Lab Clin Med 128:438-43
Shoemaker, L R; Schurman, S J; Donaldson, V H et al. (1994) Hereditary angioneurotic oedema: characterization of plasma kinin and vascular permeability-enhancing activities. Clin Exp Immunol 95:22-8
Kleniewski, J; Donaldson, V H (1993) Endothelial cells produce a substance that inhibits contact activation of coagulation by blocking the activation of Hageman factor. Proc Natl Acad Sci U S A 90:198-202
Donaldson, V H; Falconieri, M W (1993) A modification of an affinity procedure for purification of human C1- inhibitor that provides a homogeneous stable preparation. J Immunol Methods 157:101-4
Kleniewski, J; Blankenship, D T; Cardin, A D et al. (1992) Mechanism of enhanced kinin release from high molecular weight kininogen by plasma kallikrein after its exposure to plasmin. J Lab Clin Med 120:129-39
Donaldson, V H; Bissler, J J (1992) C1- inhibitors and their genes: an update. J Lab Clin Med 119:330-3
Donaldson, V H; Bernstein, D I; Wagner, C J et al. (1992) Angioneurotic edema with acquired C1- inhibitor deficiency and autoantibody to C1- inhibitor: response to plasmapheresis and cytotoxic therapy. J Lab Clin Med 119:397-406
Donaldson, V H; Mitchell, B H; Everson, B et al. (1990) Interactions of C1(-)-inhibitors from normal persons and patients with type II hereditary angioneurotic edema with purified activated Hageman factor (factor XIIa). Blood 75:911-21

Showing the most recent 10 out of 19 publications