Abstract

This program is concerned with the relation between the development of hypertension and altered electrolyte exchange of vascular smooth muscle. The specific areas of investigation related to hypertensive mechanisms are: (a) altered ion transport, (b) effects of transmural pressure, (c) small artery changes, (d) bioregulation, (e) effects of antihypertensive therapy, (f) comparison of hypertensive models, and (g) ultrastructure of vascular smooth muscle. The areas to be investigated related to transport mechanisms in vascular and intestinal smooth muscle are: (a) Na-K transport kinetics, (b) action of bioregulants on ion fluxes, and (c) Cl transport kinetics. The methods include: (1) isotope influx and efflux techniques for in-vitro vascular strips using 42K, 24Na and 36Cl, (2) computer analysis of efflux kinetics, (3) analysis of extracellular space using 60CoEDTA, (4) ion analyses (Na, K, Ca, Mg, Cl) using flame photometry and atomic absorption spectroscopy, (5) blood pressure with both direct and indirect techniques (6) contractile tension, and (7) electron microscopy.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL015852-12
Application #
3335069
Study Section
(SSS)
Project Start
1977-09-01
Project End
1986-03-31
Budget Start
1985-04-01
Budget End
1986-03-31
Support Year
12
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Missouri-Columbia
Department
Type
Schools of Medicine
DUNS #
112205955
City
Columbia
State
MO
Country
United States
Zip Code
65211

  Publications

Yang, Yan; Jones, Allan W; Thomas, Tom R et al. (2007) Influence of sex, high-fat diet, and exercise training on potassium currents of swine coronary smooth muscle. Am J Physiol Heart Circ Physiol 293:H1553-63
Rubin, L J; Magliola, L; Feng, X et al. (2005) Metabolic activation of AMP kinase in vascular smooth muscle. J Appl Physiol 98:296-306
Franke, R; Yang, Y; Rubin, L J et al. (2004) High-fat diet alters K+-currents in porcine coronary arteries and adenosine sensitivity during metabolic inhibition. J Cardiovasc Pharmacol 43:495-503
Magliola, L; Jones, A W (1999) Size dependence of functional alterations in mesenteric arteries from the aldosterone-salt hypertensive rat. J Vasc Res 36:404-14
Jones, A W; Magliola, L; Waters, C B et al. (1998) Endothelin-1 activates phospholipases and channels at similar concentrations in porcine coronary arteries. Am J Physiol 274:C1583-91
Jones, S B; Jones, A W (1995) Phorbol ester-stimulated 6-keto-prostaglandin F1 alpha in aortas from control and aldosterone-salt rats. J Hypertens 13:723-9
Liu, Y; Jones, A W; Sturek, M (1995) Ca(2+)-dependent K+ current in arterial smooth muscle cells from aldosterone-salt hypertensive rats. Am J Physiol 269:H1246-57
Liu, Y; Jones, A W; Sturek, M (1995) Attenuated Ca2+ response to acetylcholine in endothelial cells from aorta of aldosterone-salt hypertensive rats. Am J Hypertens 8:404-8
Liu, Y; Jones, A W; Sturek, M (1994) Increased barium influx and potassium current in stroke-prone spontaneously hypertensive rats. Hypertension 23:1091-5
Jones, A W; Shukla, S D; Geisbuhler, B B (1993) Stimulation of phospholipase D activity and phosphatidic acid production by norepinephrine in rat aorta. Am J Physiol 264:C609-16

Showing the most recent 10 out of 29 publications