Abstract

We will be investigating: 1) The peptide-containing nerves of the kidney. Immunocytochemical methods will be used and substance P, somatostatin, enkephalin and vasoactive intestinal polypeptides will be investigated initially. Investigation of other peptides will follow. The relationship of the peptide-containing nerves with the monoaminergic innervation will be done by combining immunocytochemical with autoradiographic methods. 2) Neuroeffector junctions in various portions of the tubule will be investigated by lifting light micrscopic autoradiograms for ultramicrotomy and electron microscopy. 3) The macula densa using immunocytochemical methods for Tamm-Horsfall protein and morphometric methods. The long term objectives of this study are the investigation of the functional anatomy of the renal nerves and the JGA. This investigation holds relevance in regard to body fluid homeostasis and possibly in the development of hypertension.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL018340-14
Application #
3335589
Study Section
Pathology A Study Section (PTHA)
Project Start
1979-01-01
Project End
1989-06-30
Budget Start
1988-01-01
Budget End
1989-06-30
Support Year
14
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
Los Angeles County Harbor-UCLA Medical Center
Department
Type
DUNS #
City
Torrance
State
CA
Country
United States
Zip Code
90509

  Publications

Barajas, L (1997) Cell-specific protein and gene expression in the juxtaglomerular apparatus. Clin Exp Pharmacol Physiol 24:520-6
Salido, E C; Lakshmanan, J; Fisher, D A et al. (1991) Expression of epidermal growth factor in the rat kidney. An immunocytochemical and in situ hybridization study. Histochemistry 96:65-72
Salido, E C; Yen, P H; Barajas, L et al. (1990) Steroid sulfatase expression in human placenta: immunocytochemistry and in situ hybridization study. J Clin Endocrinol Metab 70:1564-7
Salido, E C; Lakshmanan, J; Koy, S et al. (1990) Effect of thyroxine administration on the expression of epidermal growth factor in the kidney and submandibular gland of neonatal mice. An immunocytochemical and in situ hybridization study. Endocrinology 127:2263-9
Salido, E C; Lakshmanan, J; Shapiro, L J et al. (1990) Expression of epidermal growth factor in the kidney and submandibular gland during mouse postnatal development. An immunocytochemical and in situ hybridization study. Differentiation 45:38-43
Barajas, L; Powers, K (1990) Monoaminergic innervation of the rat kidney: a quantitative study. Am J Physiol 259:F503-11
Barajas, L; Powers, K (1989) Innervation of the renal proximal convoluted tubule of the rat. Am J Anat 186:378-88
Salido, E C; Yen, P H; Shapiro, L J et al. (1989) In situ hybridization of prepro-epidermal growth factor mRNA in the mouse kidney. Am J Physiol 256:F632-8
Barajas, L; Powers, K V (1988) Innervation of the thick ascending limb of Henle. Am J Physiol 255:F340-8
Salido, E C; Yen, P H; Shapiro, L J et al. (1988) In situ hybridization of nerve growth factor mRNA in the mouse submandibular gland. Lab Invest 59:625-30

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