The primary objective of this research project is to deepen our understanding of platelet metabolism so that the quality of platelets stored in vitro can be further improved for clinical transfusion. Within the project, there are three specific aims. The first specific aim is to more completely define the patterns of substrate selection and metabolite production by platelets. Substrates which will be studied include glucose, pyruvate, amino acids, ketone bodies, and fatty acids. The experiments will be performed on freshly collected platelets in buffer at physiological temperature and on platelets during in vitro long-term storage at 22 degrees C. The levels of activity of crucial enzymes involved in various metabolic pathways will be measured. For the second specific aim, the investigator will evaluate the use of the buffy coat method to prepare platelet concentrates. Paired studies will be performed to determine which component of the new method is responsible for producing the favorable results reported previously. For the third specific aim, megakaryocytes will be isolated from the bone marrow of guinea pigs to study their enzyme levels and patterns of substrate selection and metabolite production. The goal is to compare and contrast the metabolic patterns and enzymatic profiles of megakaryocytes with those of platelets.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Project (R01)
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Hematology Subcommittee 2 (HEM)
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Thomas Jefferson University
Internal Medicine/Medicine
Schools of Medicine
United States
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Murphy, S (1995) The oxidation of exogenously added organic anions by platelets facilitates maintenance of pH during their storage for transfusion at 22 degrees C. Blood 85:1929-35
Murphy, S; Shimizu, T; Miripol, J (1995) Platelet storage for transfusion in synthetic media: further optimization of ingredients and definition of their roles. Blood 86:3951-60
Shimizu, T; Murphy, S (1993) Roles of acetate and phosphate in the successful storage of platelet concentrates prepared with an acetate-containing additive solution. Transfusion 33:304-10
Bertolini, F; Murphy, S; Rebulla, P et al. (1992) Role of acetate during platelet storage in a synthetic medium. Transfusion 32:152-6
Murphy, S; Munoz, S; Parry-Billings, M et al. (1992) Amino acid metabolism during platelet storage for transfusion. Br J Haematol 81:585-90
Murphy, S; Kagen, L; Holme, S et al. (1991) Platelet storage in synthetic media lacking glucose and bicarbonate. Transfusion 31:16-20
Edenbrandt, C M; Murphy, S (1990) Adenine and guanine nucleotide metabolism during platelet storage at 22 degrees C. Blood 76:1884-92
Cesar, J; DiMinno, G; Alam, I et al. (1987) Plasma free fatty acid metabolism during storage of platelet concentrates for transfusion. Transfusion 27:434-7
Murphy, S (1986) Use of an arithmetic model for evaluation of in vivo platelet survival. Transfusion 26:26-7
Di Minno, G; Cerbone, A; Usberti, M et al. (1986) Platelet dysfunction in uremia. II. Correction by arachidonic acid of the impaired exposure of fibrinogen receptors by adenosine diphosphate or collagen. J Lab Clin Med 108:246-52

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