In many pathological conditions, there is an imbalance in the partition of fluid and solutes between the vascular and extravascular spaces. Changes in the hydration of the interstitial gel matrix will effect the distribution of plasma proteins between these two compartments and alter the fluid distribution. The general aim of the proposed study is to determine changes in the distribution and transport of plasma proteins in the interstitium of skin and skeletal muscle and relate the data to the present concepts on the control of fluid balance in the body. My previous studies indicate that the extravascular distribution of plasma proteins in skin and skeletal muscle following saline expansion or plasmapheresis is different from that following increased venous pressure or increased capillary wall permeability. This may have important consequences on the fluid balance between the vascular and interstitial spaces since the major fraction of the total body interstitial fluid is represented by skin and skeletal muscle. I propose to test the hypothesis that plasma proteins are carried directly from plasma to lymph through free fluid channels within the interstitium following venous congestion or altered endothelial permeability and through the interstitial gel matrix following plasmapheresis. I will study this in both skin and skeletal muscle in the hind leg of anesthetized rabbits. I will compare the equilibration rate for tracer albumin in the interstitium to that in lymph to determine whether albumin is transported through free fluid channels or through the gel matrix. I will also determine the effects of different forms of edema on electrostatic exclusion of albumin. This will be done by studying the extravascular transport and distribution of tracer albumin, which has an altered charge but not size. Additional studies will compare the effects of hyperproteinemia to those of hypoproteinemia on the extravascular distribution of fluid and plasma proteins in skin and skeletal muscle. These results will provide information on the role of the interstitial matrix in tissue fluid balance as well as provide insight into the etiology of edema formation.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL026807-07
Application #
3338715
Study Section
Cardiovascular and Pulmonary Research B Study Section (CVB)
Project Start
1980-12-01
Project End
1987-11-30
Budget Start
1986-12-01
Budget End
1987-11-30
Support Year
7
Fiscal Year
1987
Total Cost
Indirect Cost
Name
Albany Medical College
Department
Type
Schools of Medicine
DUNS #
City
Albany
State
NY
Country
United States
Zip Code
12208
Kreienberg, P B; Vincent, P A; Bell, D R et al. (1994) Isoproterenol decreases protein permeability in edematous isolated rabbit lungs: estimation of PS and sigma. J Appl Physiol 77:325-31
Kupinski, A M; Shah, D M; Bell, D R (1993) Transvascular albumin flux in rabbit hindlimb after tourniquet ischemia. Am J Physiol 264:H901-8
Wallace, J R; Bell, D R (1992) Transvascular albumin and IgG flux in skin after a continuous 3-h bradykinin infusion. Am J Physiol 263:H1064-70
Gandhi, R R; Bell, D R (1992) Importance of charge on transvascular albumin transport in skin and skeletal muscle. Am J Physiol 262:H999-1008
Wallace, J R; Bell, D R (1992) Comparison of protein lymph flux and extravascular uptake in skin during increased venous pressure. Am J Physiol 263:H895-902
Gandhi, R R; Bell, D R (1992) Influence of saline infusions on transvascular protein transport. Am J Physiol 262:H443-50
Wallace, J R; Powers, M R; Bell, D R (1992) Transvascular albumin and IgG flux in skin and skeletal muscle following plasmapheresis. Microvasc Res 44:295-306
Vincent, P A; Kreienberg, P B; Minnear, F L et al. (1992) Simultaneous measurement of fluid and protein permeability in isolated rabbit lungs during edema. J Appl Physiol 73:2440-7
Mullins, R J; Tahamont, M V; Bell, D R et al. (1991) Effect of fluid resuscitation from endotoxin shock on lung transvascular fluid and protein exchange. Am J Physiol 260:H1415-23
Powers, M R; Bell, D R (1990) Initial equilibration of albumin and IgG in rabbit hind paw skin and lymph. Microvasc Res 40:230-45

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