Abstract

Cultured adult bovine aortic endothelial cells will be used to study the mechanisms involved in maintaining the integrity of the endothelium. Because of the importance of the extracellular matrix for endothelial integrity, a study of matrix-endothelial cell interactions is proposed. The effects of matrix and matrix components on endothelial migration in vitro will be studied. Fibronectin is a major component of the matrix, and its role in the synthesis and ultrastructure of marix will be studied. Finally, conditions which alter or inhibit matrix synthesis will be developed and then used to study subsequent effects on endothelial cells.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL026838-08
Application #
3338755
Study Section
Pathobiochemistry Study Section (PBC)
Project Start
1980-04-01
Project End
1991-06-30
Budget Start
1988-07-01
Budget End
1989-06-30
Support Year
8
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
Sanford-Burnham Medical Research Institute
Department
Type
DUNS #
009214214
City
La Jolla
State
CA
Country
United States
Zip Code
92037

  Publications

Languino, L R; Ruoslahti, E (1992) An alternative form of the integrin beta 1 subunit with a variant cytoplasmic domain. J Biol Chem 267:7116-20
Gahmberg, C G; Nortamo, P; Zimmermann, D et al. (1991) The human leukocyte-adhesion ligand, intercellular-adhesion molecule 2. Expression and characterization of the protein. Eur J Biochem 195:177-82
Kirchhofer, D; Gailit, J; Ruoslahti, E et al. (1990) Cation-dependent changes in the binding specificity of the platelet receptor GPIIb/IIIa. J Biol Chem 265:18525-30
Kirchhofer, D; Languino, L R; Ruoslahti, E et al. (1990) Alpha 2 beta 1 integrins from different cell types show different binding specificities. J Biol Chem 265:615-8
Languino, L R; Gehlsen, K R; Wayner, E et al. (1989) Endothelial cells use alpha 2 beta 1 integrin as a laminin receptor. J Cell Biol 109:2455-62
Salonen, E M; Vaheri, A; Pollanen, J et al. (1989) Interaction of plasminogen activator inhibitor (PAI-1) with vitronectin. J Biol Chem 264:6339-43
Plow, E F; Pierschbacher, M D; Ruoslahti, E et al. (1987) Arginyl-glycyl-aspartic acid sequences and fibrinogen binding to platelets. Blood 70:110-5
Pytela, R; Pierschbacher, M D; Ginsberg, M H et al. (1986) Platelet membrane glycoprotein IIb/IIIa: member of a family of Arg-Gly-Asp--specific adhesion receptors. Science 231:1559-62
Plow, E F; Pierschbacher, M D; Ruoslahti, E et al. (1985) The effect of Arg-Gly-Asp-containing peptides on fibrinogen and von Willebrand factor binding to platelets. Proc Natl Acad Sci U S A 82:8057-61
Ill, C R; Ruoslahti, E (1985) Association of thrombin-antithrombin III complex with vitronectin in serum. J Biol Chem 260:15610-5

Showing the most recent 10 out of 11 publications