Abstract

Endothelial cell seeding of small caliber vascular prostheses in baboons has been shown to promote formation of an endothelial lining and to decrease platelet reactivity after two weeks. Best results were obtained with large innocula of endothelial cells after amplification in cell culture. In the continuation we propose to improve the attachment and growth of endothelial cells on vascular prostheses. The surfaces of the grafts will be coated with fibronectin, different types of collagen, and monoclonal antibodies (to endothelial cells, W6/32) to promote endothelial cell attachment and growth. The effect on endothelial cell attachment and growth will be assessed in vitro. Further evaluation will be done using an in vitro circuit and an ex vivo shunt. The most promising grafts will be studied by carotid implantation in baboons. Enhancement of endothelial cell attachment and growth may result in improved performance of small caliber vascular prostheses. The results of the above studies can be extended to human endothelial cells. The effect on attachment and growth of human endothelial cells on grafts in vitro will be investigated.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
2R01HL028855-04A1
Application #
3340103
Study Section
Surgery and Bioengineering Study Section (SB)
Project Start
1982-07-01
Project End
1989-03-31
Budget Start
1986-04-01
Budget End
1987-03-31
Support Year
4
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
Tufts University
Department
Type
DUNS #
City
Boston
State
MA
Country
United States
Zip Code
02111

  Publications

Coleman, J E; McEnroe, C S; Gelfand, J A et al. (1991) Complement activation by vascular sutures both alone and in combination with synthetic vascular prostheses. Eur J Vasc Surg 5:287-90
Verdon, S L; Chaikof, E L; Coleman, J E et al. (1990) Scanning electron microscopy analysis of polyethylene oxide hydrogels for blood contact. Scanning Microsc 4:341-9;discussion 349-50
Callow, A D (1990) The vascular endothelial cell as a vehicle for gene therapy. J Vasc Surg 11:793-8
Chaikof, E L; Coleman, J E; Ramberg, K et al. (1989) Development and evaluation of a new polymeric material for small caliber vascular prostheses. J Surg Res 47:193-9
Warner, S J; Friedman, G B; Libby, P (1989) Regulation of major histocompatibility gene expression in human vascular smooth muscle cells. Arteriosclerosis 9:279-88
Connolly, R; McEnroe, C S; Li, S et al. (1988) The effect of suture material on platelet deposition onto prosthetic material. ASAIO Trans 34:874-7
Coleman, J E; Ramberg, K; McEnroe, C S et al. (1988) Pulmonary emboli from blood-biomaterial interaction. ASAIO Trans 34:882-6
Shoenfeld, N A; Yeager, A; Connolly, R et al. (1988) A new primate model for the study of intravenous thrombotic potential and its modification. J Vasc Surg 8:49-54
Foxall, T L; Auger, K R; Callow, A D et al. (1986) Adult human endothelial cell coverage of small-caliber Dacron and polytetrafluoroethylene vascular prostheses in vitro. J Surg Res 41:158-72
Shepard, A D; Eldrup-Jorgensen, J; Keough, E M et al. (1986) Endothelial cell seeding of small-caliber synthetic grafts in the baboon. Surgery 99:318-26

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