The purpose of this proposal is to illuminate the role that membrane-bound peptides play in the overall regulation of the brain angiotensin system. The proposal utilizes a multidisciplinary approach to investigate this problem and as such includes biochemical, behavioral, electrophysiological, and immunocytochemical studies. Several specific questions related to the overall theme are addressed. These include the following: 1) What is the active form of brain angiotensin? 2) What are the functional and biochemical characteristics of membrane-bound angiotensinases? 3) What are the biochemical characteristics of solubilized and purified angiotensin receptors and are they associated with peptidase activity? 4) Can modifications of peptidase activity alter the response of angiotensin-dependent effector mechanisms? And 5) When angiotensin degradation is blocked, does brain angiotensin turnover reflect the physiological state of the animal?

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL032063-05
Application #
3343294
Study Section
Experimental Cardiovascular Sciences Study Section (ECS)
Project Start
1984-07-01
Project End
1991-06-30
Budget Start
1989-07-01
Budget End
1990-06-30
Support Year
5
Fiscal Year
1989
Total Cost
Indirect Cost
Name
Washington State University
Department
Type
Schools of Veterinary Medicine
DUNS #
041485301
City
Pullman
State
WA
Country
United States
Zip Code
99164
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Miller-Wing, A V; Hanesworth, J M; Sardinia, M F et al. (1993) Central angiotensin IV binding sites: distribution and specificity in guinea pig brain. J Pharmacol Exp Ther 266:1718-26

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