Injury to vascular endothelium results in the rapid adhesion of platelets to the exposed underlying subendothelium, an important step in the processes of hemostasis and atherogenesis. This platelet adhesion requires the presence of von Willebrand factor, a multimeric plasma protein, which we have demonstrated also to be present within vascular subendothelium. We plan to identify the subendothelial binding vessel digestion studies, by examining the binding of VWF to purified known subendothelial substituents and by immuno-electron microscopic localization techniques. We also wish to determine whether there is a multimeric preferentiality in the binding of VWF to subendothelial constitutents. Elucidation of the binding of VWF to subendothelium would be an important advance in understanding the mechanism of platelet adhesion.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL032200-03
Application #
3343507
Study Section
Hematology Subcommittee 2 (HEM)
Project Start
1984-04-01
Project End
1989-03-31
Budget Start
1986-04-01
Budget End
1989-03-31
Support Year
3
Fiscal Year
1986
Total Cost
Indirect Cost
Name
Mount Sinai School of Medicine
Department
Type
Schools of Medicine
DUNS #
City
New York
State
NY
Country
United States
Zip Code
10029
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Ross, J M; McIntire, L V; Moake, J L et al. (1995) Platelet adhesion and aggregation on human type VI collagen surfaces under physiological flow conditions. Blood 85:1826-35
Zangari, M; Kaplan, K L; Glanville, R W et al. (1995) Reduced thrombogenicity of type VI collagen as compared to type I collagen. Thromb Res 79:429-36

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