The presence of decaboxylase enzymes within the kidney permits the intrarenal conversion of amino acids to biogenic amines with potent physioligic effects. Present investigations will focus on one such enzyme-amino-acid decarboxylase. The investigations will be conducted in isolated perfused rat kidneys, anesthetized rats in vivo and chronic rat models of renal insufficiency and salt-sensitive hypertension. These techniques will be used to determine the ability of kidneys to form dopamine and and serotonin and assess their intrarenal role in the regulation of renal hemodynamics and function. Interrelationships of these renal biogenic amines with the renin-angiotensin system and prostanoids will be determined. The role of the kidney in regulating extrarenal biogenic amine formationwill be assessed. Also, it will be determined if abnormalities or renal dopamine and/or serotonin formation may play a role in salt-sensitive hypertension.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL032844-03
Application #
3344359
Study Section
Cardiovascular and Pulmonary Research B Study Section (CVB)
Project Start
1985-09-30
Project End
1988-09-29
Budget Start
1987-09-30
Budget End
1988-09-29
Support Year
3
Fiscal Year
1987
Total Cost
Indirect Cost
Name
New York Medical College
Department
Type
Schools of Medicine
DUNS #
City
Valhalla
State
NY
Country
United States
Zip Code
10595
Stier Jr, C T; Itskovitz, H D; Chen, Y H (1993) Urinary dopamine and sodium excretion in spontaneously hypertensive rats. Clin Exp Hypertens 15:105-23
Itskovitz, H D; Werber, J L; Sheridan, A M et al. (1989) 5-Hydroxytryptophan and carbidopa in spontaneously hypertensive rats. J Hypertens 7:311-5
Ding, X R; Stier Jr, C T; Itskovitz, H D (1989) Serotonin and 5-hydroxytryptophan on blood pressure and renal blood flow in anesthetized rats. Am J Med Sci 297:290-3
Itskovitz, H D; Chen, Y H; Stier Jr, C T (1988) Reciprocal renal effects of dopamine and 5-hydroxytryptamine formed within the rat kidney. Clin Sci (Lond) 75:503-7
Lam, B K; Stier Jr, C T; Wynn, N C et al. (1986) Dopamine and L-dopa potentiation of pressor responses to norepinephrine in isolated perfused rat kidneys. J Cardiovasc Pharmacol 8:554-8