Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL034704-08
Application #
2217631
Study Section
Physical Biochemistry Study Section (PB)
Project Start
1985-09-01
Project End
1996-12-31
Budget Start
1995-01-01
Budget End
1996-12-31
Support Year
8
Fiscal Year
1995
Total Cost
Indirect Cost
Name
Harvard University
Department
Biochemistry
Type
Schools of Medicine
DUNS #
082359691
City
Boston
State
MA
Country
United States
Zip Code
02115
Ehlers, M R; Schwager, S L; Chubb, A J et al. (1997) Proteolytic release of membrane proteins: studies on a membrane-protein-solubilizing activity in CHO cells. Immunopharmacology 36:271-8
Yu, X C; Sturrock, E D; Wu, Z et al. (1997) Identification of N-linked glycosylation sites in human testis angiotensin-converting enzyme and expression of an active deglycosylated form. J Biol Chem 272:3511-9
Sturrock, E D; Danilov, S M; Riordan, J F (1997) Limited proteolysis of human kidney angiotensin-converting enzyme and generation of catalytically active N- and C-terminal domains. Biochem Biophys Res Commun 236:16-9
Sturrock, E D; Yu, X C; Wu, Z et al. (1996) Assignment of free and disulfide-bonded cysteine residues in testis angiotensin-converting enzyme: functional implications. Biochemistry 35:9560-6
Ehlers, M R; Schwager, S L; Scholle, R R et al. (1996) Proteolytic release of membrane-bound angiotensin-converting enzyme: role of the juxtamembrane stalk sequence. Biochemistry 35:9549-59
Riordan, J F (1995) Angiotensin II: biosynthesis, molecular recognition, and signal transduction. Cell Mol Neurobiol 15:637-51
Ehlers, M R; Scholle, R R; Riordan, J F (1995) Proteolytic release of human angiotensin-converting enzyme expressed in Chinese hamster ovary cells is enhanced by phorbol ester. Biochem Biophys Res Commun 206:541-7
Ehlers, M R; Chen, Y N; Riordan, J F (1991) Spontaneous solubilization of membrane-bound human testis angiotensin-converting enzyme expressed in Chinese hamster ovary cells. Proc Natl Acad Sci U S A 88:1009-13
Ehlers, M R; Chen, Y N; Riordan, J F (1991) Purification and characterization of recombinant human testis angiotensin-converting enzyme expressed in Chinese hamster ovary cells. Protein Expr Purif 2:1-9
Ehlers, M R; Riordan, J F (1991) Angiotensin-converting enzyme: zinc- and inhibitor-binding stoichiometries of the somatic and testis isozymes. Biochemistry 30:7118-26

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