Abstract

Kidney contains renin and kallikrein bound to membranes. The enzymes are present in a plasma membrane enriched fraction obtained after differential centrifugation of the homogenized kidney cortex. The endoplasmic reticulum enriched fraction, however, also contains both enzymic activities. We will isolate the membranes the enzymes are bound to and establish the characteristics of the enzymes released from the membranes. We will study the process of activation and solubilization of renin and kallikrein and the various stimuli that lead to the release of the hypotensive and hypertensive enzymes.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
7R01HL036081-01
Application #
3350702
Study Section
Cardiovascular and Pulmonary Research B Study Section (CVB)
Project Start
1985-09-01
Project End
1988-08-31
Budget Start
1985-09-01
Budget End
1987-08-31
Support Year
1
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Illinois at Chicago
Department
Type
Schools of Medicine
DUNS #
121911077
City
Chicago
State
IL
Country
United States
Zip Code
60612

  Publications

Dragovic, T; Minshall, R; Jackman, H L et al. (1996) Kininase II-type enzymes. Their putative role in muscle energy metabolism. Diabetes 45 Suppl 1:S34-7
Abe, M; Nakamura, F; Tan, F et al. (1995) Expression of rat kallikrein and epithelial polarity in transfected Madin-Darby canine kidney cells. Hypertension 26:891-8
Dragovic, T; Deddish, P A; Tan, F et al. (1994) Increased expression of neprilysin (neutral endopeptidase 24.11) in rat and human hepatocellular carcinomas. Lab Invest 70:107-13
Deddish, P A; Wang, J; Michel, B et al. (1994) Naturally occurring active N-domain of human angiotensin I-converting enzyme. Proc Natl Acad Sci U S A 91:7807-11
Tan, F; Morris, P W; Skidgel, R A et al. (1993) Sequencing and cloning of human prolylcarboxypeptidase (angiotensinase C). Similarity to both serine carboxypeptidase and prolylendopeptidase families. J Biol Chem 268:16631-8
Jackman, H L; Morris, P W; Rabito, S F et al. (1993) Inactivation of endothelin-1 by an enzyme of the vascular endothelial cells. Hypertension 21:925-8
Tan, F; Weerasinghe, D K; Skidgel, R A et al. (1990) The deduced protein sequence of the human carboxypeptidase N high molecular weight subunit reveals the presence of leucine-rich tandem repeats. J Biol Chem 265:13-9
Erdos, E G (1990) Angiotensin I converting enzyme and the changes in our concepts through the years. Lewis K. Dahl memorial lecture. Hypertension 16:363-70
Erdos, E G; Skidgel, R A (1990) Renal metabolism of angiotensin I and II. Kidney Int Suppl 30:S24-7
Deddish, P A; Skidgel, R A; Kriho, V B et al. (1990) Carboxypeptidase M in Madin-Darby canine kidney cells. Evidence that carboxypeptidase M has a phosphatidylinositol glycan anchor. J Biol Chem 265:15083-9

Showing the most recent 10 out of 18 publications