Concepts concerning those factors which regulate low density lipoprotein (LDL) concentration composition and metabolism are particularly complex since this lipoprotein class contains at least two major subclasses. LDL heterogeneity or poly-dispersed LDL is of considerable interest because small more dense LDL have been associated with increased atherosclerosis and hyperthyroidism in human subjects. Utilizing a monkey model, the cebus monkey, for which we can isolate two low density lipoproteins, the influence of dietary fat saturation on LDL subclass concentration and composition will be determined. In vivo studies of the catabolism of these LDL subclasses as well as investigation of the metabolism of very low density lipoproteins as a possible precursor of these LDL subclasses will be undertaken. An in vitro liver perfusion system will be utilized to assess direct hepatic formation of LDL subclasses. In vitro studies of cellular binding, uptake and internalization of these LDL subclasses by specific cell types will also be investigated. LDL subclass concentration and composition will be correlated with the degree of atherosclerosis in the cebus monkey.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL036200-03
Application #
3350969
Study Section
Nutrition Study Section (NTN)
Project Start
1985-09-01
Project End
1989-08-31
Budget Start
1987-09-01
Budget End
1989-08-31
Support Year
3
Fiscal Year
1987
Total Cost
Indirect Cost
Name
University of Massachusetts Lowell
Department
Type
Sch Allied Health Professions
DUNS #
956072490
City
Lowell
State
MA
Country
United States
Zip Code
01854
Puppione, D L; Nicolosi, R J; Kowala, M C et al. (1989) Low density lipoprotein heterogeneity in the cebus monkey. J Lipid Res 30:641-50
Chong, K S; Nicolosi, R J; Rodger, R F et al. (1987) Effect of dietary fat saturation on plasma lipoproteins and high density lipoprotein metabolism of the rhesus monkey. J Clin Invest 79:675-83
Hegsted, D M; Nicolosi, R J (1987) Individual variation in serum cholesterol levels. Proc Natl Acad Sci U S A 84:6259-61
Cladaras, C; Hadzopoulou-Cladaras, M; Avila, R et al. (1986) Complementary DNA derived structure of the amino-terminal domain of human apolipoprotein B and size of its messenger RNA transcript. Biochemistry 25:5351-7