The long term objective of this project is knowledge of the mechanisms of regulation of smooth muscle cell differentiation. Modulation of the smooth muscle cell phenotype, in vivo is associated with wound repair, trauma, and cardiovascular diseases. We propose to use a cell culture system to study gene expression, in smooth muscle cells, as they undergo phenotype modulation. We propose to test the hypothesis that modulation is mediated by extracellular factors that are synthesized and secreted by smooth muscle cells. The factors act in an autocrine manner initiate gene activation. Particular emphasis is on the glycoprotein fibronectin, a secreted glycoprotein (38k Da protein), and an activity (nodule stimulating activity) that may have relevance to this problem. Analysis of the genes coding for those proteins to understand the regulation of the modulation process.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
1R01HL040417-01A3
Application #
3357602
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1990-04-01
Project End
1993-03-31
Budget Start
1990-04-01
Budget End
1991-03-31
Support Year
1
Fiscal Year
1990
Total Cost
Indirect Cost
Name
State University of New York at Albany
Department
Type
Schools of Arts and Sciences
DUNS #
City
Albany
State
NY
Country
United States
Zip Code
12222