Abstract

The central hypothesis of this proposal is that several important diseases of the heart manifest by altering the function of the myocardial microvasculature. The investigators plan to use the 17 frame per second cine-CT mode of the Imatron CT scanner to measure the time density curves in the myocardium in several animal models. The major emphasis in this proposal is the mathematical modeling of the shape of the time density curve. The first specific aim of this proposal is to characterize the CT method's ability to quantitate individual microvascular functional components. The investigators will embolize vessels in the heart of selected caliber using microspheres of different diameters ranging from 10 -300 micrometers, in order to impair myocardial performance.
The second aim i s to evaluate the specificity and sensitivity of the CT-based method for detection and discrimination of disease states in animal models of coronary atherosclerosis.
This aim will be studied using three groups of pigs, 28 pigs in each group; (a) hypercholesterolemia-induced pigs, (b) chronic coronary artery stenosis induced by intercoronary stenting and, (c) control subjects. The investigators propose that the contrast for these studies can be injected interveneously, and therefore the technique will be less invasive than intra-arterial coronary angiography.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL043025-07
Application #
2872895
Study Section
Diagnostic Radiology Study Section (RNM)
Project Start
1991-01-01
Project End
2001-01-31
Budget Start
1999-02-01
Budget End
2000-01-31
Support Year
7
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
Mayo Clinic, Rochester
Department
Type
DUNS #
City
Rochester
State
MN
Country
United States
Zip Code
55905

  Publications

Malyar, Nasser M; Lerman, Lilach O; Gossl, Mario et al. (2011) Relationship between surface area of nonperfused myocardium and extravascular extraction of contrast agent following coronary microembolization. Am J Physiol Regul Integr Comp Physiol 301:R430-7
Malyar, Nasser M; Gossl, Mario; Beighley, Patricia E et al. (2004) Relationship between arterial diameter and perfused tissue volume in myocardial microcirculation: a micro-CT-based analysis. Am J Physiol Heart Circ Physiol 286:H2386-92
Malyar, Nasser M; Lerman, Lilach O; Gossl, Mario et al. (2004) Relation of nonperfused myocardial volume and surface area to left ventricular performance in coronary microembolization. Circulation 110:1946-52
Mohlenkamp, Stefan; Beighley, Patricia E; Pfeifer, Eric A et al. (2003) Intramyocardial blood volume, perfusion and transit time in response to embolization of different sized microvessels. Cardiovasc Res 57:843-52
Eusemann, Christian D; Ritman, Erik L; Robb, Richard A (2003) Parametric visualization methods for the quantitative assessment of myocardial motion. Acad Radiol 10:66-76
Mohlenkamp, S; Behrenbeck, T R; Lerman, A et al. (2001) Coronary microvascular functional reserve: quantification of long-term changes with electron-beam CT preliminary results in a porcine model. Radiology 221:229-36
Mohlenkamp, S; Lerman, L O; Lerman, A et al. (2000) Minimally invasive evaluation of coronary microvascular function by electron beam computed tomography. Circulation 102:2411-6
Lerman, L O; Siripornpitak, S; Maffei, N L et al. (1999) Measurement of in vivo myocardial microcirculatory function with electron beam CT. J Comput Assist Tomogr 23:390-8
Ritman, E L (1998) Temporospatial heterogeneity of myocardial perfusion and blood volume in the porcine heart wall. Ann Biomed Eng 26:519-25
Pao, Y C; Ritman, E L (1998) Comparative characterization of the infarcted and reperfused ventricular wall muscles by finite element analysis and a myocardial muscle-blood composite model. Comput Biomed Res 31:18-31

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