The recently described class of phosphatidylinositol-anchored membrane surface proteins includes proteins which act as enzymes, receptors for cell-cell interactions, and regulators of complement activation on cell surfaces. Cromer-related antigens, which constitute polymorphisms of decay accelerating factor, comprise the first human erythrocyte blood group known to reside on a phosphatidylinositol-linked protein. Other blood group antigens--including JMH, Holly/Gregory, and Cartwright--also appear to reside on phosphatidylinositol-linked proteins. At least some of these blood group antigens, including Cromer and JMH, are also expressed on leukocytes. This application proposes to study structure and functional effects of blood group antigen-related polymorphisms of decay accelerating factor. In addition, the protein and genetic abnormalities associated with the Inab (Cromer null) and Dr(a-) (Cromer-weak) phenotypes will be investigated. Serum decay accelerating factor will also be investigated, and the abnormal protein expressed by individuals with the Inab phenotype will be characterized both structurally and functionally. Other blood group antigens which reside on and functionally. Other blood group antigens which reside on phosphatidylinositol-linked proteins will be studied, and the specific proteins bearing the antigens will be identified. As the functional roles of these proteins are identified, the effect of polymorphisms on function will also be investigated.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
1R01HL044042-01
Application #
3362768
Study Section
Hematology Subcommittee 2 (HEM)
Project Start
1990-01-15
Project End
1994-12-31
Budget Start
1990-01-15
Budget End
1990-12-31
Support Year
1
Fiscal Year
1990
Total Cost
Indirect Cost
Name
Duke University
Department
Type
Schools of Medicine
DUNS #
071723621
City
Durham
State
NC
Country
United States
Zip Code
27705
Udani, M; Rao, N; Telen, M J (1997) Leukocyte phenotypic changes in an in vitro model of ABO hemolytic transfusion reaction. Transfusion 37:904-9
Telen, M J (1995) Blood group antigens on complement receptor/regulatory proteins. Transfus Med Rev 9:20-8
Rao, N; Udani, M; Nelson, J et al. (1995) Investigations using a novel monoclonal antibody to the glycosylphosphatidylinositol-anchored protein that carries Gregory, Holley, and Dombrock blood group antigens. Transfusion 35:459-64
Mudad, R; Rao, N; Angelisova, P et al. (1995) Evidence that CDw108 membrane protein bears the JMH blood group antigen. Transfusion 35:566-70
Telen, M J (1995) Glycosyl phosphatidylinositol-linked blood group antigens and paroxysmal nocturnal hemoglobinuria. Transfus Clin Biol 2:277-90
Mudad, R; Rao, N; Issitt, P D et al. (1995) JMH variants: serologic, clinical, and biochemical analyses in two cases. Transfusion 35:925-30
Telen, M J (1995) Lutheran antigens, CD44-related antigens, and Lutheran regulatory genes. Transfus Clin Biol 2:291-301
Telen, M J (1995) Erythrocyte blood group antigens: not so simple after all. Blood 85:299-306
Telen, M J; Rao, N; Udani, M et al. (1994) Molecular mapping of the Cromer blood group Cra and Tca epitopes of decay accelerating factor: toward the use of recombinant antigens in immunohematology. Blood 84:3205-11
Telen, M J; Rao, N (1994) Recent advances in immunohematology. Curr Opin Hematol 1:143-50

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