The long term goal is to extend the storage shelf life of human red cells (RBC) with improved in vivo survival. An additive-preservative solution containing ammonium (NH4+) and phosphate (Pi) ions in addition to adenine, glucose, and mannitol has been shown to maintain adenosine triphosphate (ATP) levels of RBC high enough for more than 12 weeks at 4 to suggest good in vivo RBC survivals.
The aims are to find the survival studies to establish the maximum storage interval compatible with acceptable posttransfusion survival; to perform metabolic studies to establish the biochemical pathways involved in the maintenance of high ATP concentrations under these conditions. Metabolic studies are designed to determine whether increased synthesis of AMP or its decreased degradation of both occur. The role of phosphofructokinase activity will also be explored. Preliminary data indicate a strong possibility that satisfactory storage of RBC for 12 or more weeks can be achieved. The resulting blood donor programs; conservation of the blood supply by decreased loss through outdating; and decreasing the usage of blood and the rate of tissue iron accumulation in chronically transfused patients because of the longer in vivo survival of better preserved RBC.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL044897-02
Application #
3363686
Study Section
Hematology Subcommittee 2 (HEM)
Project Start
1990-07-01
Project End
1993-06-30
Budget Start
1991-07-01
Budget End
1992-06-30
Support Year
2
Fiscal Year
1991
Total Cost
Indirect Cost
Name
University of Cincinnati
Department
Type
Schools of Medicine
DUNS #
City
Cincinnati
State
OH
Country
United States
Zip Code
45221
Dumaswala, U J; Wilson, M J; Jose, T et al. (1997) Effect of a glycerol-containing hypotonic medium on erythrocyte phospholipid asymmetry and aminophospholipid transport during storage. Biochim Biophys Acta 1330:265-73
Tse, L C; Dumaswala, U J; Greenwalt, T J (1996) Separation of mixed red cell populations by using microbead columns. J Lab Clin Med 127:489-93
Greenwalt, T J; Dumaswala, U J; Rugg, N (1996) Studies in red blood cell preservation 10. 51Cr recovery of red cells after liquid storage in a glycerol-containing additive solution. Vox Sang 70:6-10
Dumaswala, U J; Wilson, M J; Jose, T et al. (1996) Glutamine- and phosphate-containing hypotonic storage media better maintain erythrocyte membrane physical properties. Blood 88:697-704
Greenwalt, T J (1995) The Ernest Witebsky memorial lecture. Red but not dead: not a hapless sac of hemoglobin. Immunol Invest 24:3-21
Dumaswala, U J; Sukati, H; Greenwalt, T J (1995) The protein composition of red cell eluates. Transfusion 35:33-6
Greenwalt, T J; Dumaswala, U J; Dhingra, N et al. (1993) Studies in red blood cell preservation. 7. In vivo and in vitro studies with a modified phosphate-ammonium additive solution. Vox Sang 65:87-94
Dumaswala, U J; Petrosky, T L; Greenwalt, T J (1992) Studies in red blood cell preservation. 6. Red cell membrane remodeling during rejuvenation. Vox Sang 63:12-5
Dumaswala, U J; Oreskovic, R T; Petrosky, T L et al. (1992) Studies in red blood cell preservation. 5. Determining the limiting concentrations of NH4Cl and Na2HPO4 needed to maintain red blood cell ATP during storage. Vox Sang 62:136-40
Greenwalt, T J; McGuinness, C G; Dumaswala, U J (1991) Studies in red blood cell preservation: 4. Plasma vesicle hemoglobin exceeds free hemoglobin. Vox Sang 61:14-7