This proposal concerns a widely distributed soluble angiotensin-binding protein that was originally discovered and purified. This protein probably mediates some of the actions of angiotensin II and the knowledge of its structure and regulation should be useful for understanding the mechanism of action of the octapeptide and for devising angiotensin antagonists as possible antihypertensive agents.
The specific aims of the proposal are: 1.To determine the primary structure of the soluble rabbit hepatic binding protein by cloning and sequencing the cDNA that encodes it. 2.To examine regulation of the similar soluble, immunologically homologous binding protein in cultured murine NlE-115 cells. These cells exhibit functional responses to angiotensin II and also contain well characterized angiotensin receptors on their membranes that are physiologically regulated. Emphasis will be on determining whether the regulation and/or structure of the soluble and membrane receptors is related; whether the functional effects of angiotensin II can be dissociated based on the properties of the two types of receptors; and whether the soluble protein can be physiologically recruited to and from membranes.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
1R01HL045313-01
Application #
3364296
Study Section
Cardiovascular and Renal Study Section (CVB)
Project Start
1990-07-01
Project End
1993-06-30
Budget Start
1990-07-01
Budget End
1991-06-30
Support Year
1
Fiscal Year
1990
Total Cost
Indirect Cost
Name
Weill Medical College of Cornell University
Department
Type
Schools of Medicine
DUNS #
201373169
City
New York
State
NY
Country
United States
Zip Code
10065