Cyclosporin A (CsA) is a cyclic undecapeptide with selective immunosuppressive properties. It is widely used in humans to prevent allograft rejection of kidney, liver, heart, lung and bone marrow transplants. Most recently it has been found to inhibit the multiplication of HIV--1 (the causative agent of AIDS) A major objective of the ongoing research in this laboratory has been to clarify the basis of its unique immunosuppressive activity by identifying and eventually isolating cell receptors with which it interacts. Since CsA is also cytotoxic after prolonged administration it is of particular interest to distinguish interactions that lead to toxicity from those responsible for immunosuppression. This laboratory has recently identified a CsA receptor on the surface of lymphocytes that is associated with immunosuppression. This proposal describes the methods that we will use to isolate and characterize this receptor.
The Specific Aims of this proposal are: 1. To isolate and purify the putative receptor and examine its interaction with CsA and with various lymphokines. 2. To obtain antibodies to the purified receptor and to test them for biologic activity. 3. To clone the receptor, exploiting the findings obtained in the pursuit of Specific Aims 2 and 3, and to compare its sequence with that of known receptors.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
1R01HL047377-01A3
Application #
2223601
Study Section
Immunological Sciences Study Section (IMS)
Project Start
1994-12-01
Project End
1997-11-30
Budget Start
1994-12-01
Budget End
1995-11-30
Support Year
1
Fiscal Year
1995
Total Cost
Indirect Cost
Name
Columbia University (N.Y.)
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
167204994
City
New York
State
NY
Country
United States
Zip Code
10032
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