Oligosaccharide Assembly on Recombinant Proteins

Castellino, Francis J.

Abstract

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Heart, Lung, and Blood Institute (NHLBI)
• Type: Research Project (R01)
• Project #: 5R01HL049172-04
• Application #: 2225275
• Study Section: Physiological Chemistry Study Section (PC)
• Project Start: 1993-08-01
• Project End: 1997-10-31
• Budget Start: 1996-08-01
• Budget End: 1997-10-31
• Support Year: 4
• Fiscal Year: 1996

Institution

• Name: University of Notre Dame
• Department: Chemistry
• Type: Schools of Arts and Sciences
• DUNS #: 824910376
• City: Notre Dame
• State: IN
• Country: United States
• Zip Code: 46556

Publications

Ren, J; Castellino, F J; Bretthauer, R K (1997) Purification and properties of alpha-mannosidase II from Golgi-like membranes of baculovirus-infected Spodoptera frugiperda (IPLB-SF-21AE) cells. Biochem J 324 ( Pt 3):951-6

Miele, R G; Nilsen, S L; Brito, T et al. (1997) Glycosylation properties of the Pichia pastoris-expressed recombinant kringle 2 domain of tissue-type plasminogen activator. Biotechnol Appl Biochem 25 ( Pt 2):151-7

Miele, R G; Castellino, F J; Bretthauer, R K (1997) Characterization of the acidic oligosaccharides assembled on the Pichia pastoris-expressed recombinant kringle 2 domain of human tissue-type plasminogen activator. Biotechnol Appl Biochem 26 ( Pt 2):79-83

Ren, J; Bretthauer, R K; Castellino, F J (1995) Purification and properties of a Golgi-derived (alpha 1,2)-mannosidase-I from baculovirus-infected lepidopteran insect cells (IPLB-SF21AE) with preferential activity toward mannose6-N-acetylglucosamine2. Biochemistry 34:2489-95

Basu, S S; Dastgheib-Hosseini, S; Hoover, G et al. (1994) Analysis of glycosphingolipids by fluorophore-assisted carbohydrate electrophoresis using ceramide glycanase from Mercenaria mercenaria. Anal Biochem 222:270-4