Having cloned the gene for the Duffy protein, the Investigators propose to: 1. determine the topology of the amino and carboxyl ends by immunochemical binding or double labeling immunoelectromicroscopy (an approach that is not intuitively obvious; 2. determine the antibody and malarial binding sites on the external portion of the molecule; 3. determine the sequence of non- erythroid Duffy protein; 4. determine by immunochemistry which cells produce Duffy proteins; 5. determine the chemikine binding requirements; 6. find and clone the mouse equivalent gene; and 7. study the interactions of Duffy and Rh since a Duffy antigen (Fy5) depends upon the presence of Rh proteins for its expression. Long-term goals consist of the following: 1. determination of the function of the glycoprotein; 2. determine the significance of a Duffy like protein in the brain; 3. determine the sequence the binds to the merozoites of malaria organisms; 4. determine whether the Duffy antigen is a transporter; and 5. design mouse knock-out models to determine the significance of the Duffy protein.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
1R01HL053297-01A1
Application #
2231144
Study Section
Hematology Subcommittee 2 (HEM)
Project Start
1995-08-01
Project End
1998-07-31
Budget Start
1995-08-01
Budget End
1996-07-31
Support Year
1
Fiscal Year
1995
Total Cost
Indirect Cost
Name
New York Blood Center
Department
Type
DUNS #
City
New York
State
NY
Country
United States
Zip Code
10065