Abstract

The Applicant notes that after transplantation stem cell (SC) renewal is very limited as indicated by the reduced concentration of SC for second and third retransplantations. It is hypothesized that this is either due to prior treatment which may damage the marrow stroma or to the disruption of the marrow with harvest.
The aim of the proposal is therefore to study self-renewal of SC under these conditions and alternate conditions. He will work with two separate populations of SC, rhodamine-123 (Rh-123low), a dull-stain population that is non cycling but capable of mediating long-term reconstitution and Rh-123hi cells, a brightly staining population which mediates short-term reconstitution of hematopoietic cells. Studies done in vitro have already shown that the Rh-123low cells can differentiate into Rh-123hi cells.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL056857-03
Application #
2750580
Study Section
Hematology Subcommittee 2 (HEM)
Project Start
1996-08-01
Project End
2000-07-31
Budget Start
1998-08-01
Budget End
1999-07-31
Support Year
3
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
University of Utah
Department
Pathology
Type
Schools of Medicine
DUNS #
City
Salt Lake City
State
UT
Country
United States
Zip Code
84112

  Publications

Hughes, Alisa K; Schmid, Douglas I; Kohan, Donald E (2002) Sex steroids do not affect shigatoxin cytotoxicity on human renal tubular or glomerular cells. BMC Nephrol 3:6
Slayton, William B; Georgelas, Ann; Pierce, L Jeanne et al. (2002) The spleen is a major site of megakaryopoiesis following transplantation of murine hematopoietic stem cells. Blood 100:3975-82
Hughes, A K; Stricklett, P K; Kohan, D E (2001) Shiga toxin-1 regulation of cytokine production by human glomerular epithelial cells. Nephron 88:14-23
Schmid, D I; Kohan, D E (2001) Effect of shigatoxin-1 on arachidonic acid release by human glomerular epithelial cells. Kidney Int 60:1026-36
Mojica, M P; Perry, S S; Searles, A E et al. (2001) Phenotypic distinction and functional characterization of pro-B cells in adult mouse bone marrow. J Immunol 166:3042-51
Wiesmann, A; Kim, M; Georgelas, A et al. (2000) Modulation of hematopoietic stem/progenitor cell engraftment by transforming growth factor beta. Exp Hematol 28:128-39
Spangrude, G J; Cooper, D D (2000) Paradigm shifts in stem-cell biology. Semin Hematol 37:10-Mar
Hughes, A K; Stricklett, P K; Schmid, D et al. (2000) Cytotoxic effect of Shiga toxin-1 on human glomerular epithelial cells. Kidney Int 57:2350-9
Wiesmann, A; Phillips, R L; Mojica, M et al. (2000) Expression of CD27 on murine hematopoietic stem and progenitor cells. Immunity 12:193-9
Searles, A E; Pohlmann, S J; Pierce, L J et al. (2000) Rapid, B lymphoid-restricted engraftment mediated by a primitive bone marrow subpopulation. J Immunol 165:67-74

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