The applicant proposes to determine if augmentation of 7alpha-hydroxylase activity in the liver or ectopic expression of 7alpha-hydroxylase in macrophages has a favorable effect on atherogenesis in hyperlipidemic mice.
The first aim i s to determine how transgenic expression of 7alpha-hydroxylase in the liver of hyperlipidemic mice influences plasma lipoprotein levels and atherogenesis.
The second aim i s to determine if the transgenic expression of 7alpha-hydroxylase in macrophages of hyperlipidemic mice influences atherogenesis. In other studies, bone marrow of hyperlipidemic mice will be replaced with stem cells obtained from mice expressing a 7alpha-hydroxylase macrophage-specific transgene.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL057974-03
Application #
2910643
Study Section
Nutrition Study Section (NTN)
Project Start
1997-05-01
Project End
2002-04-30
Budget Start
1999-06-15
Budget End
2000-04-30
Support Year
3
Fiscal Year
1999
Total Cost
Indirect Cost
Name
San Diego State University
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
073371346
City
San Diego
State
CA
Country
United States
Zip Code
92182
Bradshaw, Gary; Gutierrez, Alejandra; Miyake, Jon H et al. (2005) Facilitated replacement of Kupffer cells expressing a paraoxonase-1 transgene is essential for ameliorating atherosclerosis in mice. Proc Natl Acad Sci U S A 102:11029-34