Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
1R01HL058249-01
Application #
2031451
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
1996-09-30
Project End
2001-09-29
Budget Start
1996-09-30
Budget End
1997-09-29
Support Year
1
Fiscal Year
1996
Total Cost
Indirect Cost
Name
Carnegie-Mellon University
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
052184116
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213
Chang, Chung-ke; Simplaceanu, Virgil; Ho, Chien (2002) Effects of amino acid substitutions at beta 131 on the structure and properties of hemoglobin: evidence for communication between alpha 1 beta 1- and alpha 1 beta 2-subunit interfaces. Biochemistry 41:5644-55
Tsai, Ching-Hsuan; Ho, Chien (2002) Recombinant hemoglobins with low oxygen affinity and high cooperativity. Biophys Chem 98:15-25
Tsai, C H; Fang, T Y; Ho, N T et al. (2000) Novel recombinant hemoglobin, rHb (beta N108Q), with low oxygen affinity, high cooperativity, and stability against autoxidation. Biochemistry 39:13719-29
Fang, T Y; Simplaceanu, V; Tsai, C H et al. (2000) An additional H-bond in the alpha 1 beta 2 interface as the structural basis for the low oxygen affinity and high cooperativity of a novel recombinant hemoglobin (beta L105W). Biochemistry 39:13708-18
Tsai, C H; Shen, T J; Ho, N T et al. (1999) Effects of substitutions of lysine and aspartic acid for asparagine at beta 108 and of tryptophan for valine at alpha 96 on the structural and functional properties of human normal adult hemoglobin: roles of alpha 1 beta 1 and alpha 1 beta 2 subunit inter Biochemistry 38:8751-61
Jeong, S T; Ho, N T; Hendrich, M P et al. (1999) Recombinant hemoglobin(alpha 29leucine --> phenylalanine, alpha 96valine --> tryptophan, beta 108asparagine --> lysine) exhibits low oxygen affinity and high cooperativity combined with resistance to autoxidation. Biochemistry 38:13433-42
Puius, Y A; Zou, M; Ho, N T et al. (1998) Novel water-mediated hydrogen bonds as the structural basis for the low oxygen affinity of the blood substitute candidate rHb(alpha 96Val-->Trp). Biochemistry 37:9258-65
Huang, S; Peterson, E S; Ho, C et al. (1997) Quaternary structure sensitive tyrosine interactions in hemoglobin: a UV resonance Raman study of the double mutant rHb (beta99Asp-->Asn, alpha42Tyr-->Asp). Biochemistry 36:6197-206