Abstract

A major goal of the human genome project is identification of the complete set of human genes. Single-pass, partial sequencing of cDNA clones to generate ESTs provides a rapid and cost-effective method of gene discovery. At the same time, sequence information contained within ESTs provides a key resource for gene mapping. Understanding the function of newly identified human genes requires the increased use of model organisms that can be genetically manipulated. The generation of ESTs from model organisms and comparative genome mapping of these ESTs can also be exploited for human disease gene hunting, as well as investigating the large-scale structure and organization of the genome. The rat is an important experimental model for studying the genetic basis of many human diseases and a wide array of genetically bred rat strains with disease characteristics are available. Hence, it would be beneficial to generate an EST catalog and unique gene index of The rat genome. This application proposes the following specific aims: (1) Generate a rat EST catalog (REST) by randomly sampling cDNA clones from normalized rat cDNA libraries of different tissues. (2) Construct non- normalized full-length rat cDNA libraries and employ micro array technology.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL059781-02
Application #
2771647
Study Section
Special Emphasis Panel (ZHL1-CSR-B (SA))
Project Start
1997-09-30
Project End
2001-02-28
Budget Start
1998-09-25
Budget End
2001-02-28
Support Year
2
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
Institute for Genomic Research
Department
Type
DUNS #
City
Rockville
State
MD
Country
United States
Zip Code
20850

  Publications

Goutsias, J; Lee, N H (2007) Computational and experimental approaches for modeling gene regulatory networks. Curr Pharm Des 13:1415-36
Irby, Rosalyn B; Malek, Renae L; Bloom, Greg et al. (2005) Iterative microarray and RNA interference-based interrogation of the SRC-induced invasive phenotype. Cancer Res 65:1814-21
Lee, Norman H (2005) Genomic approaches for reconstructing gene networks. Pharmacogenomics 6:245-58
Tollet-Egnell, Petra; Parini, Paolo; Stahlberg, Nina et al. (2004) Growth hormone-mediated alteration of fuel metabolism in the aged rat as determined from transcript profiles. Physiol Genomics 16:261-7
Malek, Renae L; Irby, Rosalyn B; Guo, Qingbin M et al. (2002) Identification of Src transformation fingerprint in human colon cancer. Oncogene 21:7256-65
Flores-Morales, Amilcar; Gullberg, Hjalmar; Fernandez, Leandro et al. (2002) Patterns of liver gene expression governed by TRbeta. Mol Endocrinol 16:1257-68
Tollet-Egnell, P; Flores-Morales, A; Stahlberg, N et al. (2001) Gene expression profile of the aging process in rat liver: normalizing effects of growth hormone replacement. Mol Endocrinol 15:308-18
Miller, L D; Park, K S; Guo, Q M et al. (2001) Silencing of Wnt signaling and activation of multiple metabolic pathways in response to thyroid hormone-stimulated cell proliferation. Mol Cell Biol 21:6626-39
Flores-Morales, A; Stahlberg, N; Tollet-Egnell, P et al. (2001) Microarray analysis of the in vivo effects of hypophysectomy and growth hormone treatment on gene expression in the rat. Endocrinology 142:3163-76
Hegde, P; Qi, R; Gaspard, R et al. (2001) Identification of tumor markers in models of human colorectal cancer using a 19,200-element complementary DNA microarray. Cancer Res 61:7792-7

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