Fanon anemia (FA) is a complex genetic disorder characterized by a progressive bone marrow aplasia, chromosomal instability, and the acquisition of malignancies, particularly myeloid leukemia. Eight complementation types (FA-A to FA-H), inferring the existence of eight genes, have been identified in FA patients. The cDNAs of the FA-A, FA-C, and FA-G genes, (FANCA), (FANCC) and (FANCG), respectively, have been identified raising the potential of using gene transfer technology to introduce the functional cDNA into autologous stem cells. Currently the only cure for the uniformly fatal hematopoietic disease in FA patients is HLA identical allogeneic bone marrow umbilical cord blood cell transplantation, a therapy available to only about 30% of patients. Previously, a major limitation in studying the pathogenesis of this hematopoietic disease was the inability to evaluate cellular phenotypes resulting from gene inactivation as well as hematopoietic function following insertion of the correct cDNA into the cell in an in vivo system. The recent development of a murine model containing a disruption of the murine homologue of FANCC (Fanc) now allows studies of these questions. The laboratory has utilized this model and determined that hematopoietic progenitors from mice that are homozygous for a disruption of FancC are hypersensitive to mitomycin c in myeloid progenitor assays similar to the mitomycin c hypersensitivity observed in progenitors cultured from bone marrow cells of FA-C patients. In addition, the investigator has shown that FanC -/- progenitors are predisposed to apoptosis in response to inhibitory cytokines. Recently, the investigator demonstrated that loss of FancC results in a decrease of the long term repopulating ability of freshly isolated bone marrow hematopoietic stem cells. The goal of the studies proposed here are to utilize this murine model to evaluate hematopoietic stem and progenitor cell function in FancC -/- hematopoietic cells, to determine whether introduction of recombinant FancC can restore normal hematopoietic progenitor and stem cell function and to determine whether normal FancC +/+ cells or genetically corrected repopulating stem cells can engraft into FancC -/- mice in the absence of myeloablation.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL063219-04
Application #
6655692
Study Section
Hematology Subcommittee 2 (HEM)
Program Officer
Thomas, John
Project Start
2000-09-10
Project End
2004-08-31
Budget Start
2003-09-01
Budget End
2004-08-31
Support Year
4
Fiscal Year
2003
Total Cost
$324,407
Indirect Cost
Name
Indiana University-Purdue University at Indianapolis
Department
Pediatrics
Type
Schools of Medicine
DUNS #
603007902
City
Indianapolis
State
IN
Country
United States
Zip Code
46202
Li, Xiaxin; Le Beau, Michelle M; Ciccone, Samantha et al. (2005) Ex vivo culture of Fancc-/- stem/progenitor cells predisposes cells to undergo apoptosis, and surviving stem/progenitor cells display cytogenetic abnormalities and an increased risk of malignancy. Blood 105:3465-71
Broxmeyer, Hal E; Orschell, Christie M; Clapp, D Wade et al. (2005) Rapid mobilization of murine and human hematopoietic stem and progenitor cells with AMD3100, a CXCR4 antagonist. J Exp Med 201:1307-18
Freie, Brian W; Ciccone, Samantha L M; Li, Xiaxin et al. (2004) A role for the Fanconi anemia C protein in maintaining the DNA damage-induced G2 checkpoint. J Biol Chem 279:50986-93
Li, Xiaxin; Yang, Yanzhu; Yuan, Jin et al. (2004) Continuous in vivo infusion of interferon-gamma (IFN-gamma) preferentially reduces myeloid progenitor numbers and enhances engraftment of syngeneic wild-type cells in Fancc-/- mice. Blood 104:1204-9
Broxmeyer, Hal E; Cooper, Scott; Kohli, Lisa et al. (2003) Transgenic expression of stromal cell-derived factor-1/CXC chemokine ligand 12 enhances myeloid progenitor cell survival/antiapoptosis in vitro in response to growth factor withdrawal and enhances myelopoiesis in vivo. J Immunol 170:421-9
Broxmeyer, Hal E; Kohli, Lisa; Kim, Chang H et al. (2003) Stromal cell-derived factor-1/CXCL12 directly enhances survival/antiapoptosis of myeloid progenitor cells through CXCR4 and G(alpha)i proteins and enhances engraftment of competitive, repopulating stem cells. J Leukoc Biol 73:630-8
Haneline, Laura S; Li, Xiaxin; Ciccone, Samantha L M et al. (2003) Retroviral-mediated expression of recombinant Fancc enhances the repopulating ability of Fancc-/- hematopoietic stem cells and decreases the risk of clonal evolution. Blood 101:1299-307
Li, Xiaxin; Plett, P Artur; Yang, Yanzhu et al. (2003) Fanconi anemia type C-deficient hematopoietic stem/progenitor cells exhibit aberrant cell cycle control. Blood 102:2081-4
Li, Binghui; Yang, Feng-Chun; Clapp, D Wade et al. (2003) Enforced expression of CUL-4A interferes with granulocytic differentiation and exit from the cell cycle. Blood 101:1769-76
Freie, Brian; Li, Xiaxin; Ciccone, Samantha L M et al. (2003) Fanconi anemia type C and p53 cooperate in apoptosis and tumorigenesis. Blood 102:4146-52

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