Abstract

MUC 1 is a transmembrane cell surface mucin best known for its upregulation in breast cancer. The PI observes that its expression correlates with goblet cell differentiation in hamster tracheal epithelial cells in vitro. His preliminary promoter analysis of the hamster Muc 1 gene cloned in his lab reveals the presence of a silencer (P-NRE) not previously described. He proposes conventional methods to (1) deduce the nucleotides comprising the silencer, (2) identify the protein binding to the silencer, (3) clone this protein and (4) perform studies to determine if and how the protein-DNA interaction mediates Muc 1 upregulation during goblet cell differentiation in vitro.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL063742-03
Application #
6499017
Study Section
Lung Biology and Pathology Study Section (LBPA)
Program Officer
Banks-Schlegel, Susan P
Project Start
2000-02-01
Project End
2004-01-31
Budget Start
2002-02-01
Budget End
2003-01-31
Support Year
3
Fiscal Year
2002
Total Cost
$180,542
Indirect Cost

  Institution

Name
University of Maryland Baltimore
Department
Other Health Professions
Type
Schools of Pharmacy
DUNS #
003255213
City
Baltimore
State
MD
Country
United States
Zip Code
21201

  Publications

Lillehoj, Erik P; Kato, Kosuke; Lu, Wenju et al. (2013) Cellular and molecular biology of airway mucins. Int Rev Cell Mol Biol 303:139-202
Kuwahara, Ippei; Lillehoj, Erik P; Lu, Wenju et al. (2006) Neutrophil elastase induces IL-8 gene transcription and protein release through p38/NF-{kappa}B activation via EGFR transactivation in a lung epithelial cell line. Am J Physiol Lung Cell Mol Physiol 291:L407-16
Chaturvedi, Rupesh; Srivastava, Rakesh K; Hisatsune, Akinori et al. (2005) Augmentation of Fas ligand-induced apoptosis by MUC1 mucin. Int J Oncol 26:1169-76
Lee, Insong J; Han, Feng; Baek, Jin et al. (2004) Inhibition of MUC1 expression by indole-3-carbinol. Int J Cancer 109:810-6
Hisatsune, Akinori; Hyun, Sang Won; Lee, Insong James et al. (2004) YY1 transcription factor is not responsible for the negative regulation of hamster Muc1 transcription. Anticancer Res 24:235-40
Kim, Kwang Chul; Hisatsune, Akinori; Kim, Do Jin et al. (2003) Pharmacology of airway goblet cell mucin release. J Pharmacol Sci 92:301-7
Lee, Insong James; Hyun, Sang Won; Nandi, Asit et al. (2003) Transcriptional regulation of the hamster Muc1 gene: identification of a putative negative regulatory element. Am J Physiol Lung Cell Mol Physiol 284:L160-8
Lillehoj, Erik R; Kim, K Chul (2002) Airway mucus: its components and function. Arch Pharm Res 25:770-80