The goal of this proposal is to develop a methodology, using near infrared (NIR) Raman spectroscopy, as a quantitative method, for in situ spectral analysis of atherosclerosis and its progression in human peripheral arteries. With this technique, information on the arterial morphology and chemical composition can be obtained rapidly, accurately and remotely. Therefore, it has the potential to provide quantitative measurement of histomorphological and histopathological markers for both the presence and extent of atherosclerotic alterations. The specific goals of the proposal are to: 1) perform basic, quantitative studies of the relationship between the Raman spectrum and the artery histochemistry and morphology. We propose several approaches for characterizing fully the spectroscopic variability of the NIR Raman signals with disease-associated alterations of artery. We have planned specific experiments to compare Raman spectra with artery morphology, and use this information to establish diagnostic decision schemes. 2) Develop a new class of highly sensitive NIR optical fiber spectral microprobes for use in operating room and clinical settings. 3) Perform clinical studies to evaluate new probe designs in vivo during peripheral vascular surgery and in the presence of interferences such as blood.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
1R01HL064675-01A1
Application #
6334290
Study Section
Biophysical Chemistry Study Section (BBCB)
Program Officer
Altieri, Frank
Project Start
2001-09-30
Project End
2004-08-31
Budget Start
2001-09-30
Budget End
2002-08-31
Support Year
1
Fiscal Year
2001
Total Cost
$277,181
Indirect Cost
Name
Massachusetts Institute of Technology
Department
Internal Medicine/Medicine
Type
Schools of Arts and Sciences
DUNS #
City
Cambridge
State
MA
Country
United States
Zip Code
02139
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