Recently, promising new MRI techniques using laser polarized aboutHe and arterial spin tagging (AST) have been developed for studying ventilation and perfusion (V/Q) in human lungs. In patients with emphysema and pulmonary emboli (PE), these techniques have produced dramatic images with the characteristic V/Q distributions of these diseases. While preliminary results indicate that this new integrated MRI technique can be used for studying regional V/Q abnormalities, the technique has not been clinically or physiologically validated. The ultimate clinical usefulness of this non-invasive imaging modality will only be possible if is validated by an established method. The main goal of this proposal is the refinement of V/Q imaging using polarized 3He and AST, and its validation in an animal model. This technique will then aid in the overall assessment of lung function and in the development of a model to quantify ventilation, perfusion, and physiologic V/Q relationships in the normal and diseased lung. We propose to validate 3He/AST method using single-photon emission computed tomography and the multiple inert gas elimination technique by accomplishing the following specific aims: 1. To establish the normal values of the regional helium distribution and perfusion signal in normal pigs. We will perform ventilation with hyperpolarized 3He and perfusion with AST. We will also perform the multiple inert gas elimination technique (MIGET) in each pig to confirm that its V/Q distributions are normal. 2. To obtain 3He ventilation and AST perfusion MRI in pigs after airway occlusion by various sizes of glass beads as an airway model for emphysema. 3. To obtain 3He ventilation and AST perfusion MRI in pigs with autologous clot inserted into their pulmonary artery as a model of PE. 4. 3He gas MRI and artenal spin-tagging perfusion scans will be used to deterrnine V/Q changes following the administration of streptokinase as treatment for the induced PE. Once validated, this integrated MRI method will provide novel anatormic and physiologic information for detection and treatment of pulmonary diseases.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
1R01HL064741-01A1
Application #
6286249
Study Section
Diagnostic Radiology Study Section (RNM)
Program Officer
Garfinkel, Susan J
Project Start
2001-02-15
Project End
2004-01-31
Budget Start
2001-02-15
Budget End
2002-01-31
Support Year
1
Fiscal Year
2001
Total Cost
$396,250
Indirect Cost
Name
University of Pennsylvania
Department
Radiation-Diagnostic/Oncology
Type
Schools of Medicine
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
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